DEREGULATION OF TYROSINE KINASES IN A RAT MACROPHAGE TUMOR - ECTOPIC EXPRESSION OF LCK
- TS SRIDHAR
- V REMA
- G SWARUP
- A KHAR
Affiliations: CTR CELLULAR & MOLEC BIOL,HYDERABAD 500007,ANDHRA PRADESH,INDIA.
- Published online on: February 1, 1995 https://doi.org/10.3892/ijo.6.2.487
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AK-5, a spontaneous rat macrophage tumor has 2-3 fold higher membrane-associated tyrosine kinase (TK) activity than normal peritoneal macrophages. lck, the lymphoid-specific TK, is expressed ectopically in AK-5. p56(lck), the protein product of lck, was detected in AK-5 by immunoprecipitation. The surface proteins CD4 and CD8 to which p56(lck) is complexed in lymphocytes are not present an the surface of AK-5. The transcripts of hck, a gene expressed predominantly in myeloid cells are markedly reduced in AK-5. The genomic organization of lck is unaltered. Although the total phosphotyrosine content of proteins is not altered significantly, there is a qualitative change in the profile of phosphotyrosine containing proteins. These results provide evidence for the ectopic expression of lck in a hematopoietic tumor and bolster the existing data implicating the participation of src family TKs in spontaneous neoplasms.