NATURAL-KILLER CELL-MEDIATED CYTOTOXICITY OF BLOOD-LYMPHOCYTES FROM PATIENTS WITH MALIGNANT MESOTHELIOMA TREATED BY INTRAPLEURAL INTERLEUKIN-2

  • Authors:
    • P ASTOUL
    • E NUSSBAUM
    • C BOUTIN
  • View Affiliations

  • Published online on: February 1, 1995     https://doi.org/10.3892/ijo.6.2.431
  • Pages: 431-436
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Abstract

The most impressive biological effect of recombinant Interleukin-2 (rIL-2) is the generation of nonspecific killer cells that have lytic activity for a variety of tumor cells. Numerous studies have shown that these non specific killer cells might be of NK cell lineage even though they are different from resident NK-cell. We have examined the kinetics of the NK cell-mediated cytotoxicity of blood lymphocytes in patients after intrapleural rIL-2 administered for the treatment of pleural cancer. Escalating doses of rIL-2 were administered by intrapleural route to treat 11 patients with malignant pleural effusions due to malignant pleural mesothelioma (4 stage I, 4 stage II, 2 stage III, 1 stage IV). Two patients received respectively 3 cycles and 2 cycles of treatment. Peripheral blood lymphocyte cytotoxicity was assessed by an in vitro, chromium release microcytotoxicity assay against K562 cell line. Preliminary results indicate: (i) an important and prolonged increase in the cytotoxic response of blood lymphocytes in all patients but one having a clinical response and (ii) a lack of cytotoxicity or a baseline cytotoxic response of blood lymphocytes in all patients but one with no clinical response. These results likely point out the significance of NK-activity in the IL-2-induced antitumoral response and the interest of this in vitro assay for screening patients for further cycles of treatment.

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February 1995
Volume 6 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
ASTOUL, P., NUSSBAUM, E., & BOUTIN, C. (1995). NATURAL-KILLER CELL-MEDIATED CYTOTOXICITY OF BLOOD-LYMPHOCYTES FROM PATIENTS WITH MALIGNANT MESOTHELIOMA TREATED BY INTRAPLEURAL INTERLEUKIN-2. International Journal of Oncology, 6, 431-436. https://doi.org/10.3892/ijo.6.2.431
MLA
ASTOUL, P., NUSSBAUM, E., BOUTIN, C."NATURAL-KILLER CELL-MEDIATED CYTOTOXICITY OF BLOOD-LYMPHOCYTES FROM PATIENTS WITH MALIGNANT MESOTHELIOMA TREATED BY INTRAPLEURAL INTERLEUKIN-2". International Journal of Oncology 6.2 (1995): 431-436.
Chicago
ASTOUL, P., NUSSBAUM, E., BOUTIN, C."NATURAL-KILLER CELL-MEDIATED CYTOTOXICITY OF BLOOD-LYMPHOCYTES FROM PATIENTS WITH MALIGNANT MESOTHELIOMA TREATED BY INTRAPLEURAL INTERLEUKIN-2". International Journal of Oncology 6, no. 2 (1995): 431-436. https://doi.org/10.3892/ijo.6.2.431