EFFECTS OF C-MYC ANTISENSE TRANSCRIPTS ON DIFFERENTIATION OF K562 CELLS

  • Authors:
    • YW YANG
    • MC YANG
  • View Affiliations

  • Published online on: February 1, 1995     https://doi.org/10.3892/ijo.6.2.419
  • Pages: 419-424
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

An antisense myc plasmid, containing a portion of exon 2 of the human c-myc gene, was cloned into the pDOL retroviral vector which was used to transfect human K562 erythroleukemia cells. All the retroviral vector transformants showed slower growth rates than the parental K562 cells. No detectable difference in the sense myc RNA level was observed, but the reduction of myc protein levels in the antisense myc transformants varied between 21% and 96%. Most antisense myc transformants contain higher percentage of hemoglobin-producing cells, but no proportionate correlation with the myc protein levels was observed. The results suggest that myc protein is necessary but not sufficient for the regulation of differentiation and growth arrest of K562 cells.

Related Articles

Journal Cover

February 1995
Volume 6 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
YANG, Y., & YANG, Y. (1995). EFFECTS OF C-MYC ANTISENSE TRANSCRIPTS ON DIFFERENTIATION OF K562 CELLS. International Journal of Oncology, 6, 419-424. https://doi.org/10.3892/ijo.6.2.419
MLA
YANG, Y., YANG, M."EFFECTS OF C-MYC ANTISENSE TRANSCRIPTS ON DIFFERENTIATION OF K562 CELLS". International Journal of Oncology 6.2 (1995): 419-424.
Chicago
YANG, Y., YANG, M."EFFECTS OF C-MYC ANTISENSE TRANSCRIPTS ON DIFFERENTIATION OF K562 CELLS". International Journal of Oncology 6, no. 2 (1995): 419-424. https://doi.org/10.3892/ijo.6.2.419