K-RAS MUTATION IN COLORECTAL CARCINOMAS FROM SINGAPORE
- TW PHANG
- CY SHI
- A WEE
- SS NGOI
- B LI
- HP LEE
- CN ONG
Affiliations: NATL UNIV SINGAPORE, DEPT COMMUNITY OCCUPAT & FAMILY MED, SINGAPORE 0511, SINGAPORE. NATL UNIV SINGAPORE, DEPT PATHOL, SINGAPORE 0511, SINGAPORE. NATL UNIV SINGAPORE, DEPT SURG, SINGAPORE 0511, SINGAPORE. NATL UNIV SINGAPORE, INST MOLEC & CELL BIOL, SINGAPORE 0511, SINGAPORE.
- Published online on: January 1, 1995 https://doi.org/10.3892/ijo.6.1.191
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54 sporadic colorectal cancers were analyzed for aberrations in the K-ras oncogene. DNA was extracted from frozen tissues obtained from surgical resection and analyzed for mutations in codons 12, 13 and 61 of the K-ras oncogene using single strand conformation polymorphism analysis (SSCP) and direct sequencing. Point mutations in the K-ras oncogene were found in 26/54 (48%) cases, all of which resulted in amino acid substitutions. No other types of mutations (e.g. insertions or deletions) were found. 4 of the mutations were at codon 12, 22 in codon 13 and only 1 was a codon 61 mutant. G-->A transitions were found to be predominant. A remarkable finding was the high preponderance of (13)Gly-(13)Ser mutations (54%). No correlation was observed between K-ras mutations and tumor location, Dukes' stage, differentiation levels, age or sex of the patient.