MODULATION OF PROLIFERATION AND CELLULAR-CHANGES OF HUMAN PROSTATIC-CANCER CELLS BY ACTIVATED LYMPHOCYTES

  • Authors:
    • TC HSIEH
    • W XU
    • SP TANG
    • JW CHIAO
  • View Affiliations

  • Published online on: October 1, 1994     https://doi.org/10.3892/ijo.5.4.979
  • Pages: 979-984
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Abstract

The effect of immune cells on the growth and development of human prostatic cancer cells was investigated. Cell proliferation of androgen-independent human prostatic cancer cells JCA-1 was reduced 30-40% with the supplementation of a growth modulating activity present in lymphocyte conditioned medium. The slower growing cells had a longer doubling time revealing a blocking for G(1) cells entering into S phase. The clonogenicity of the cells was also reduced. Displayed in parallel was altered phenotypes including the development of dendrite-like processes with less intercellular contact. Immunocytochemical staining showed significantly increased: expression of actin, vimentin and cytokeratin 8, suggesting a differentiated cell type. Activated lymphocytes were shown to require RNA synthesis for providing the modulating activity which was estimated to be 18,500-27,500 Da. The role of immune cells in growth regulation of prostatic cells is discussed.

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October 1994
Volume 5 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
HSIEH, T., XU, W., TANG, S., & CHIAO, J. (1994). MODULATION OF PROLIFERATION AND CELLULAR-CHANGES OF HUMAN PROSTATIC-CANCER CELLS BY ACTIVATED LYMPHOCYTES. International Journal of Oncology, 5, 979-984. https://doi.org/10.3892/ijo.5.4.979
MLA
HSIEH, T., XU, W., TANG, S., CHIAO, J."MODULATION OF PROLIFERATION AND CELLULAR-CHANGES OF HUMAN PROSTATIC-CANCER CELLS BY ACTIVATED LYMPHOCYTES". International Journal of Oncology 5.4 (1994): 979-984.
Chicago
HSIEH, T., XU, W., TANG, S., CHIAO, J."MODULATION OF PROLIFERATION AND CELLULAR-CHANGES OF HUMAN PROSTATIC-CANCER CELLS BY ACTIVATED LYMPHOCYTES". International Journal of Oncology 5, no. 4 (1994): 979-984. https://doi.org/10.3892/ijo.5.4.979