MODULATION OF PROLIFERATION AND CELLULAR-CHANGES OF HUMAN PROSTATIC-CANCER CELLS BY ACTIVATED LYMPHOCYTES
- TC HSIEH
- W XU
- SP TANG
- JW CHIAO
Affiliations: NEW YORK MED COLL,DEPT MED,VALHALLA,NY 10595.
- Published online on: October 1, 1994 https://doi.org/10.3892/ijo.5.4.979
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The effect of immune cells on the growth and development of human prostatic cancer cells was investigated. Cell proliferation of androgen-independent human prostatic cancer cells JCA-1 was reduced 30-40% with the supplementation of a growth modulating activity present in lymphocyte conditioned medium. The slower growing cells had a longer doubling time revealing a blocking for G(1) cells entering into S phase. The clonogenicity of the cells was also reduced. Displayed in parallel was altered phenotypes including the development of dendrite-like processes with less intercellular contact. Immunocytochemical staining showed significantly increased: expression of actin, vimentin and cytokeratin 8, suggesting a differentiated cell type. Activated lymphocytes were shown to require RNA synthesis for providing the modulating activity which was estimated to be 18,500-27,500 Da. The role of immune cells in growth regulation of prostatic cells is discussed.