CHARACTERIZATION OF A MUTATED P53 EXHIBITING A CELL CYCLE-RELATED NUCLEAR TRANSLOCATION
- P CAHEN
- D GRUNWALD
- D PIGOTT
- MP BROCARD
- S KHOCHBIN
- JJ LAWRENCE
Affiliations: CEA,DEPT BIOL MOLEC & STRUCT,INSERM,U309,BIOL MOLEC CYCLE CELLULAIRE LAB,F-38054 GRENOBLE 9,FRANCE.
- Published online on: October 1, 1994 https://doi.org/10.3892/ijo.5.4.811
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We studied the p53 expression in the F4NW0 cell line. We found that this mutated p53 has a deletion of eight amino acids, between the conserved domains IV and V. Only one of the two alleles is expressed. It contains a single base mutation, which seems to promote the use of a cryptic splicing acceptor site, resulting in the observed deletion. The protein shows a cell cycle-dependent nuclear translocation: in G(1), it is exclusively cytoplasmic, and during the G(1)/S transition, it translocates into the nucleus. Nevertheless, different antibodies assayed revealed different patterns of localization.