CHARACTERIZATION OF A MUTATED P53 EXHIBITING A CELL CYCLE-RELATED NUCLEAR TRANSLOCATION

  • Authors:
    • P CAHEN
    • D GRUNWALD
    • D PIGOTT
    • MP BROCARD
    • S KHOCHBIN
    • JJ LAWRENCE
  • View Affiliations

  • Published online on: October 1, 1994     https://doi.org/10.3892/ijo.5.4.811
  • Pages: 811-817
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Abstract

We studied the p53 expression in the F4NW0 cell line. We found that this mutated p53 has a deletion of eight amino acids, between the conserved domains IV and V. Only one of the two alleles is expressed. It contains a single base mutation, which seems to promote the use of a cryptic splicing acceptor site, resulting in the observed deletion. The protein shows a cell cycle-dependent nuclear translocation: in G(1), it is exclusively cytoplasmic, and during the G(1)/S transition, it translocates into the nucleus. Nevertheless, different antibodies assayed revealed different patterns of localization.

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October 1994
Volume 5 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
CAHEN, P., GRUNWALD, D., PIGOTT, D., BROCARD, M., KHOCHBIN, S., & LAWRENCE, J. (1994). CHARACTERIZATION OF A MUTATED P53 EXHIBITING A CELL CYCLE-RELATED NUCLEAR TRANSLOCATION. International Journal of Oncology, 5, 811-817. https://doi.org/10.3892/ijo.5.4.811
MLA
CAHEN, P., GRUNWALD, D., PIGOTT, D., BROCARD, M., KHOCHBIN, S., LAWRENCE, J."CHARACTERIZATION OF A MUTATED P53 EXHIBITING A CELL CYCLE-RELATED NUCLEAR TRANSLOCATION". International Journal of Oncology 5.4 (1994): 811-817.
Chicago
CAHEN, P., GRUNWALD, D., PIGOTT, D., BROCARD, M., KHOCHBIN, S., LAWRENCE, J."CHARACTERIZATION OF A MUTATED P53 EXHIBITING A CELL CYCLE-RELATED NUCLEAR TRANSLOCATION". International Journal of Oncology 5, no. 4 (1994): 811-817. https://doi.org/10.3892/ijo.5.4.811