PHORBOL 12-MYRISTATE 13-ACETATE (PMA) INDUCES NEUROENDOCRINE-LIKE DIFFERENTIATION AND REVERSES DOXORUBICIN-RESISTANCE OF HUMAN COLON-CARCINOMA CELLS IN-VITRO

  • Authors:
    • P CORREALE
    • M CARAGLIA
    • S PEPE
    • B RICCIARDI
    • C BARILE
    • S MONTAGNANI
    • R PACELLI
    • AR BIANCO
    • P TAGLIAFERRI
  • View Affiliations

  • Published online on: February 1, 1994     https://doi.org/10.3892/ijo.4.2.423
  • Pages: 423-427
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Abstract

Human colon carcinoma LoVo/DX cells, which have been selected from parental LoVo for resistance to doxorubicin, express a typical multidrug resistant (MDR-1) phenotype. We have investigated whether phorbol 12-myristate 13-acetate (PMA) which often induces phenotypical changes in human tumor cells could, at the same time, modulate differentiation and sensitivity of LoVo/DX cells to doxorubicin. After 48 h exposure to 100 nM PMA, morphological changes became evident on LoVo/DX cells which showed elongated cytoplasm and dendritic-like structures: moreover immunocytochemical findings were suggestive of neuroendocrine-like differentiation. Under the same experimental conditions, LoVo/DX became sensitive to doxorubicin and showed enhanced intracellular drug-accumulation and reduced membrane expression of the 170 kD glycoprotein GP-170, which is the cellular product of the mdr1 gene. We conclude that pharmacological induction of tumor cell differentiation by PMA is paralleled by abrogation of drug resistance in a colon carcinoma MDR-1 cell line.

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February 1994
Volume 4 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
CORREALE, P., CARAGLIA, M., PEPE, S., RICCIARDI, B., BARILE, C., MONTAGNANI, S. ... TAGLIAFERRI, P. (1994). PHORBOL 12-MYRISTATE 13-ACETATE (PMA) INDUCES NEUROENDOCRINE-LIKE DIFFERENTIATION AND REVERSES DOXORUBICIN-RESISTANCE OF HUMAN COLON-CARCINOMA CELLS IN-VITRO. International Journal of Oncology, 4, 423-427. https://doi.org/10.3892/ijo.4.2.423
MLA
CORREALE, P., CARAGLIA, M., PEPE, S., RICCIARDI, B., BARILE, C., MONTAGNANI, S., PACELLI, R., BIANCO, A., TAGLIAFERRI, P."PHORBOL 12-MYRISTATE 13-ACETATE (PMA) INDUCES NEUROENDOCRINE-LIKE DIFFERENTIATION AND REVERSES DOXORUBICIN-RESISTANCE OF HUMAN COLON-CARCINOMA CELLS IN-VITRO". International Journal of Oncology 4.2 (1994): 423-427.
Chicago
CORREALE, P., CARAGLIA, M., PEPE, S., RICCIARDI, B., BARILE, C., MONTAGNANI, S., PACELLI, R., BIANCO, A., TAGLIAFERRI, P."PHORBOL 12-MYRISTATE 13-ACETATE (PMA) INDUCES NEUROENDOCRINE-LIKE DIFFERENTIATION AND REVERSES DOXORUBICIN-RESISTANCE OF HUMAN COLON-CARCINOMA CELLS IN-VITRO". International Journal of Oncology 4, no. 2 (1994): 423-427. https://doi.org/10.3892/ijo.4.2.423