DETECTION OF KI-RAS MUTATION IN NONNEOPLASTIC MUCOSA OF JAPANESE PATIENTS WITH COLORECTAL CANCERS

  • Authors:
    • T MINAMOTO
    • Z RONAI
    • N YAMASHITA
    • A OCHIAI
    • T SUGIMURA
    • M MAI
    • H ESUMI
  • View Affiliations

  • Published online on: February 1, 1994     https://doi.org/10.3892/ijo.4.2.397
  • Pages: 397-401
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Abstract

About 50% of colorectal adenocarcinoma in humans have been reported to contain mutated Ki-ras gene. To provide a better understanding for the possible role of this mutation and to examine whether its presence can reliably predict a risk of colorectal cancer, we have analyzed the normal appearing mucosa of patients with colorectal adenocarcinoma. With an Enriched PCR procedure, we can detect mutated Ki-ras allele in the presence of 10(3) to 10(4) normal alleles. Only by this procedure was Ki-ras mutation detected in the non-neoplastic colonic mucosa of 9 out of 50 (18%) Japanese patients with colorectal cancer. This analysis indicated that epithelial cells which carry mutated Ki-ras gene were 100- to 1000-fold less frequent in the non-neoplastic mucosa than at the tumor site. The presence of ras gene mutation in normal appearing mucosa points to a previous exposure which had initiated the multistage process of colorectal carcinogenesis.

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February 1994
Volume 4 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
MINAMOTO, T., RONAI, Z., YAMASHITA, N., OCHIAI, A., SUGIMURA, T., MAI, M., & ESUMI, H. (1994). DETECTION OF KI-RAS MUTATION IN NONNEOPLASTIC MUCOSA OF JAPANESE PATIENTS WITH COLORECTAL CANCERS. International Journal of Oncology, 4, 397-401. https://doi.org/10.3892/ijo.4.2.397
MLA
MINAMOTO, T., RONAI, Z., YAMASHITA, N., OCHIAI, A., SUGIMURA, T., MAI, M., ESUMI, H."DETECTION OF KI-RAS MUTATION IN NONNEOPLASTIC MUCOSA OF JAPANESE PATIENTS WITH COLORECTAL CANCERS". International Journal of Oncology 4.2 (1994): 397-401.
Chicago
MINAMOTO, T., RONAI, Z., YAMASHITA, N., OCHIAI, A., SUGIMURA, T., MAI, M., ESUMI, H."DETECTION OF KI-RAS MUTATION IN NONNEOPLASTIC MUCOSA OF JAPANESE PATIENTS WITH COLORECTAL CANCERS". International Journal of Oncology 4, no. 2 (1994): 397-401. https://doi.org/10.3892/ijo.4.2.397