DETECTION OF KI-RAS MUTATION IN NONNEOPLASTIC MUCOSA OF JAPANESE PATIENTS WITH COLORECTAL CANCERS
- T MINAMOTO
- Z RONAI
- N YAMASHITA
- A OCHIAI
- T SUGIMURA
- M MAI
- H ESUMI
Affiliations: NATL CANC CTR,RES INST,DIV BIOCHEM,5-1-1 TSUKIJI,CHUO KU,TOKYO 104,JAPAN. NATL CANC CTR,RES INST,DIV PATHOL,CHUO KU,TOKYO 104,JAPAN. AMER HLTH FDN,MOLEC CARCINOGENESIS PROGRAM,VALHALLA,NY 10595. KANAZAWA UNIV,CANC RES INST,DEPT SURG,KANAZAWA,ISHIKAWA 921,JAPAN.
- Published online on: February 1, 1994 https://doi.org/10.3892/ijo.4.2.397
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About 50% of colorectal adenocarcinoma in humans have been reported to contain mutated Ki-ras gene. To provide a better understanding for the possible role of this mutation and to examine whether its presence can reliably predict a risk of colorectal cancer, we have analyzed the normal appearing mucosa of patients with colorectal adenocarcinoma. With an Enriched PCR procedure, we can detect mutated Ki-ras allele in the presence of 10(3) to 10(4) normal alleles. Only by this procedure was Ki-ras mutation detected in the non-neoplastic colonic mucosa of 9 out of 50 (18%) Japanese patients with colorectal cancer. This analysis indicated that epithelial cells which carry mutated Ki-ras gene were 100- to 1000-fold less frequent in the non-neoplastic mucosa than at the tumor site. The presence of ras gene mutation in normal appearing mucosa points to a previous exposure which had initiated the multistage process of colorectal carcinogenesis.