MACROPHAGE INFLAMMATORY PROTEIN (MIP)-1-ALPHA STEM-CELL INHIBITOR (SCI) DOES NOT AFFECT CLONAL GROWTH OF HUMAN SOLID TUMOR-CELL LINES IN-VITRO

  • Authors:
    • A KORFEL
    • Z VONMARSCHALL
    • M KOENIGSMANN
    • D OBERBERG
    • B REUFI
    • E THIEL
    • WE BERDEL
  • View Affiliations

  • Published online on: February 1, 1994     https://doi.org/10.3892/ijo.4.2.353
  • Pages: 353-357
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Abstract

MIP-1alpha is a member of a family of proinflammatory cytokines produced by activated macrophages which has been shown to be a negative regulator of early hematopoietic stem cell progenitors. We report on results testing recombinant human (rh) MIP-1alpha on the clonal growth of different human nonhematopoietic tumor cell lines in vitro. Cell lines tested included the following histologies: 7 glioblastomas, 1 neuroblastoma, 2 head and neck carcinomas, 4 lung carcinomas, 3 colorectal carcinomas, 1 gastric carcinoma, 1 pancreatic carcinoma, 1 breast carcinoma, 1 prostate carcinoma, 1 choriocarcinoma, 1 ovary carcinoma, 1 osteosarcoma, and 3 melanomas. MIP-1alpha (0, 2, 20, 200 ng/ml) was tested in human tumor cloning assays (HTCA) in agar-containing capillaries (HTCAcap) and in mixtures of methylcellulose and agar (HTCAmix), representing assay systems with different plating efficiencies (PE). Tumor cells were continuously exposed to the cytokine for the complete assay period. Clonal growth of none of the cell lines was significantly and reproducibly stimulated or inhibited by MIP-1alpha.

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February 1994
Volume 4 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
KORFEL, A., VONMARSCHALL, Z., KOENIGSMANN, M., OBERBERG, D., REUFI, B., THIEL, E., & BERDEL, W. (1994). MACROPHAGE INFLAMMATORY PROTEIN (MIP)-1-ALPHA STEM-CELL INHIBITOR (SCI) DOES NOT AFFECT CLONAL GROWTH OF HUMAN SOLID TUMOR-CELL LINES IN-VITRO. International Journal of Oncology, 4, 353-357. https://doi.org/10.3892/ijo.4.2.353
MLA
KORFEL, A., VONMARSCHALL, Z., KOENIGSMANN, M., OBERBERG, D., REUFI, B., THIEL, E., BERDEL, W."MACROPHAGE INFLAMMATORY PROTEIN (MIP)-1-ALPHA STEM-CELL INHIBITOR (SCI) DOES NOT AFFECT CLONAL GROWTH OF HUMAN SOLID TUMOR-CELL LINES IN-VITRO". International Journal of Oncology 4.2 (1994): 353-357.
Chicago
KORFEL, A., VONMARSCHALL, Z., KOENIGSMANN, M., OBERBERG, D., REUFI, B., THIEL, E., BERDEL, W."MACROPHAGE INFLAMMATORY PROTEIN (MIP)-1-ALPHA STEM-CELL INHIBITOR (SCI) DOES NOT AFFECT CLONAL GROWTH OF HUMAN SOLID TUMOR-CELL LINES IN-VITRO". International Journal of Oncology 4, no. 2 (1994): 353-357. https://doi.org/10.3892/ijo.4.2.353