EXPRESSION OF PROTEIN-KINASE-C ISOZYMES IN MULTIDRUG-RESISTANT MURINE LEUKEMIA P388/ADR CELLS

  • Authors:
    • S GUPTA
    • C KIM
    • YF QIN
    • S GOLLAPUDI
  • View Affiliations

  • Published online on: February 1, 1994     https://doi.org/10.3892/ijo.4.2.311
  • Pages: 311-315
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Abstract

Because of a suggested role of protein kinase C (PKC) in multidrug resistance (MDR) and recent molecular cloning of PKC isozymes, we compared the expression of Ca++-dependent PKCalpha. beta and gamma, and Ca++-independent PKCdelta, epsilon, and zeta isozymes between drug sensitive murine leukemia P388 and its MDR subline P388/ADR, using qualitative and quantitative polymerase chain reaction (PCR) and Western blot techniques. The expression of PKCalpha and PKCbeta mRNA and their proteins was higher in P388/ADR as compared to P388 cells. In contrast, PKCdelta, epsilon, zeta mRNA were decreased in P388/ADR cells as compared to P388 cells. However, at the protein level the expression of PKCdelta, epsilon, and zeta was also increased in P388/ADR cells as compared to P388 cells, suggesting an increased rate of translation of PKCdelta, epsilon, and zeta isozymes in P388/ADR cells. No PKCgamma isozyme was detected by PCR and Western blot analyses. Confocal microscopic examination revealed a distinct pattern of subcellular distribution of PKCbeta isozymes in P388/ADR when compared with P388 cells. This study demonstrates the presence of altered levels of PKC isozymes in P388/ADR cells that may suggest a role of certain PKC isozymes in MDR.

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February 1994
Volume 4 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
GUPTA, S., KIM, C., QIN, Y., & GOLLAPUDI, S. (1994). EXPRESSION OF PROTEIN-KINASE-C ISOZYMES IN MULTIDRUG-RESISTANT MURINE LEUKEMIA P388/ADR CELLS. International Journal of Oncology, 4, 311-315. https://doi.org/10.3892/ijo.4.2.311
MLA
GUPTA, S., KIM, C., QIN, Y., GOLLAPUDI, S."EXPRESSION OF PROTEIN-KINASE-C ISOZYMES IN MULTIDRUG-RESISTANT MURINE LEUKEMIA P388/ADR CELLS". International Journal of Oncology 4.2 (1994): 311-315.
Chicago
GUPTA, S., KIM, C., QIN, Y., GOLLAPUDI, S."EXPRESSION OF PROTEIN-KINASE-C ISOZYMES IN MULTIDRUG-RESISTANT MURINE LEUKEMIA P388/ADR CELLS". International Journal of Oncology 4, no. 2 (1994): 311-315. https://doi.org/10.3892/ijo.4.2.311