Reduced T-cadherin expression and promoter methylation are associated with the development and progression of hepatocellular carcinoma

  • Authors:
    • Qun Yan
    • Zhi-Fa Zhang
    • Xiao-Ping Chen
    • David H. Gutmann
    • Min Xiong
    • Zhen-Yu Xiao
    • Zhi-Yong Huang
  • View Affiliations

  • Published online on: May 1, 2008     https://doi.org/10.3892/ijo.32.5.1057
  • Pages: 1057-1063
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Abstract

Loss of T-cadherin expression has been reported in a number of human cancers. We previously reported that T-cadherin re-expression suppressed cell growth and motility in glioma. Here, we report that the T-cadherin expression was significantly decreased in human hepatocellular carcinoma (HCC) compared to adjacent normal liver. In addition, T-cadherin expression in HCC with metastasis was significantly lower than in HCC without metastasis. To determine the mechanism underlying the reduced T-cadherin expression in HCC, we examined T-cadherin promoter methylation. We found that methylation of the T-cadherin promoter was present in 40% of HCC, but absent in all adjacent liver tissues. In the HCC with T-cadherin promoter methylation, the T-cadherin expression was significantly decreased compared to HCC without methylation. To provide a functional link between T-cadherin promoter methylation and T-cadherin growth regulation, we used the HepG2 hepatoma cell line that exhibits T-cadherin promoter methylation. Treatment of HepG2 cells with the demethylating agent 5-aza-2-deoxycytidine resulted in increased T-cadherin expression and reduced cell proliferation. These results demonstrate that the T-cadherin down-regulation by promoter methylation is associated with the development and progression of HCC, and suggest that T-cadherin is an important tumor suppressor in liver cancer.

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May 2008
Volume 32 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Yan, Q., Zhang, Z., Chen, X., Gutmann, D.H., Xiong, M., Xiao, Z., & Huang, Z. (2008). Reduced T-cadherin expression and promoter methylation are associated with the development and progression of hepatocellular carcinoma. International Journal of Oncology, 32, 1057-1063. https://doi.org/10.3892/ijo.32.5.1057
MLA
Yan, Q., Zhang, Z., Chen, X., Gutmann, D. H., Xiong, M., Xiao, Z., Huang, Z."Reduced T-cadherin expression and promoter methylation are associated with the development and progression of hepatocellular carcinoma". International Journal of Oncology 32.5 (2008): 1057-1063.
Chicago
Yan, Q., Zhang, Z., Chen, X., Gutmann, D. H., Xiong, M., Xiao, Z., Huang, Z."Reduced T-cadherin expression and promoter methylation are associated with the development and progression of hepatocellular carcinoma". International Journal of Oncology 32, no. 5 (2008): 1057-1063. https://doi.org/10.3892/ijo.32.5.1057