Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer

  • Authors:
    • G. J. Byrne
    • K. E. Hayden
    • G. McDowell
    • H. Lang
    • C. C. Kirwan
    • L. Tetlow
    • S. Kumar
    • N. J. Bundred
  • View Affiliations

  • Published online on: November 1, 2007     https://doi.org/10.3892/ijo.31.5.1127
  • Pages: 1127-1132
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Abstract

In cancer models, thrombospondin-1 (TSP-1) has been shown to inhibit angiogenesis or promote metastasis by increasing adhesion of malignant cells to endothelium. To determine the role of TSP-1 in breast cancer and breast cancer angiogenesis, we have measured TSP-1 in plasma and tumour cytosols and compared levels to established clinicopathological prognostic parameters and intratumoural microvessel density. TSP-1 was measured, by radioimmunoassay, in plasma (pTSP-1) and tumour cytosols (cTSP-1) of women with early breast cancer (EBC) (n=71). pTSP-1 in EBC was compared to pTSP-1 levels in women with advanced breast cancer (ABC) (n=66), normal controls (n=77) and was correlated with prognostic features and microvessel density (MVD) (measured by CD31 immunostaining). cTSP-1 levels were compared to prognostic features and microvessel density. pTSP-1 in women with EBC (median 484, IQR 344-877 ng/ml) and ABC (median 588, IQR 430-952 ng/ml) were elevated when compared to normal controls (median 21, IQR 175-247) (p<0.001). Women with lymph node metastases (n=35) had higher levels of TSP-1 (median 799 ng/ml, IQR 455-943) than women who were node negative (median 343 ng/ml, IQR 267-514) (n=36) (p<0.05). Levels of pTSP-1 in EBC correlated with MVD (R=0.39, p<0.05). Levels of TSP-1 in tumour cytosols of women with EBC (median 1714, IQR 893-5283 ng/ml) correlated with microvessel density (R=0.46, p<0.01). Circulating levels of TSP-1 appear to be a marker of breast cancer aggressiveness and in breast cancer may have a pro-angiogenic rather than anti-angiogenic role.

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November 2007
Volume 31 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Byrne, G.J., Hayden, K.E., McDowell, G., Lang, H., Kirwan, C.C., Tetlow, L. ... Bundred, N.J. (2007). Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer. International Journal of Oncology, 31, 1127-1132. https://doi.org/10.3892/ijo.31.5.1127
MLA
Byrne, G. J., Hayden, K. E., McDowell, G., Lang, H., Kirwan, C. C., Tetlow, L., Kumar, S., Bundred, N. J."Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer". International Journal of Oncology 31.5 (2007): 1127-1132.
Chicago
Byrne, G. J., Hayden, K. E., McDowell, G., Lang, H., Kirwan, C. C., Tetlow, L., Kumar, S., Bundred, N. J."Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer". International Journal of Oncology 31, no. 5 (2007): 1127-1132. https://doi.org/10.3892/ijo.31.5.1127