Short hairpin RNA targeting survivin inhibits growth and angiogenesis of glioma U251 cells

  • Authors:
    • Hai-Ning Zhen
    • Li-Wen Li
    • Wei Zhang
    • Zhou Fei
    • Chang-Hong Shi
    • Tong-Tao Yang
    • Wen-Tao Bai
    • Xiang Zhang
  • View Affiliations

  • Published online on: November 1, 2007     https://doi.org/10.3892/ijo.31.5.1111
  • Pages: 1111-1117
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Abstract

Survivin is a novel tumor-associated gene, its overexpression mostly associates with carcinogenesis and development. Nevertheless, the precise role of survivin in initiation and progression of gliomas is still not completely clear. We constructed here three short hairpin RNA (shRNA) targeting survivin plasmid vectors and introduced them into glioma U251 cells. The three shRNAs were efficiently and specifically able to knockdown the survivin expression in transiently transfected U251 cells. The stable transfectants expressing the shRNA having the strongest inhibitory effect against survivin exhibited decreased cell growth, increased spontaneous apoptosis, mitotic catastrophe and cell cycle arrest. Furthermore, in nude mice xenografts, the stable transfectants presented decreased de novo glioma formation and reduced development of angiogenesis. Results from this study indicate that survivin plays an important role in malignant proliferation, antiapoptosis and angiogenesis of gliomas, which may become an attractive target for gene therapy of gliomas, while RNA interference (RNAi) mediated by shRNA may become a new promising strategy for cancer gene therapy.

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November 2007
Volume 31 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Zhen, H., Li, L., Zhang, W., Fei, Z., Shi, C., Yang, T. ... Zhang, X. (2007). Short hairpin RNA targeting survivin inhibits growth and angiogenesis of glioma U251 cells. International Journal of Oncology, 31, 1111-1117. https://doi.org/10.3892/ijo.31.5.1111
MLA
Zhen, H., Li, L., Zhang, W., Fei, Z., Shi, C., Yang, T., Bai, W., Zhang, X."Short hairpin RNA targeting survivin inhibits growth and angiogenesis of glioma U251 cells". International Journal of Oncology 31.5 (2007): 1111-1117.
Chicago
Zhen, H., Li, L., Zhang, W., Fei, Z., Shi, C., Yang, T., Bai, W., Zhang, X."Short hairpin RNA targeting survivin inhibits growth and angiogenesis of glioma U251 cells". International Journal of Oncology 31, no. 5 (2007): 1111-1117. https://doi.org/10.3892/ijo.31.5.1111