Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells

  • Authors:
    • Michihide Shimono
    • Akiko Uenaka
    • Yuji Noguchi
    • Shuichiro Sato
    • Hideo Okumura
    • Kazuhiko Nakagawa
    • Katsuyuki Kiura
    • Mitsune Tanimoto
    • Eiichi Nakayama
  • View Affiliations

  • Published online on: April 1, 2007     https://doi.org/10.3892/ijo.30.4.835
  • Pages: 835-840
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Abstract

We previously demonstrated a dominant IgG response against XAGE-1b antigen in a lung cancer patient by serological analysis of antigens by recombinant expression cloning (SEREX) analysis using a cDNA library from the autologous OU-LU-6 tumor cell line. In this study, we investigated recognition of XAGE-1b on OU-LU-6 tumor by the patient CD4-expressing tumor-infiltrating lymphocytes (CD4 TIL). The response of CD4 TIL obtained from malignant pleural effusion was determined against autologous OU-LU-6 tumor cells and XAGE-1b mRNA-transfected PHA-stimulated CD4 T-cells (T-APC) from healthy individuals sharing HLA-DR with the patient by performing IFNγ secretion and ELISPOT assays. The patient CD4 TIL recognized OU-LU-6 in an HLA-DR-restricted manner and XAGE-1b mRNA-transfected T-APC derived from DRB1∗0901-sharing healthy donor (HD)1 and HD2, but not DRB1∗1101-sharing HD3 or HD4. Epitope analysis using 17 18-mer peptides with 12 overlapping amino acids revealed that the CD4 TIL recognized XAGE-1b 33-49. The findings suggest that the patient CD4 T-cells recognized the XAGE-1b 33-49-related epitope on autologous OU-LU-6 tumor cells in a manner restricted by DR∗0901.

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April 2007
Volume 30 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Shimono, M., Uenaka, A., Noguchi, Y., Sato, S., Okumura, H., Nakagawa, K. ... Nakayama, E. (2007). Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells. International Journal of Oncology, 30, 835-840. https://doi.org/10.3892/ijo.30.4.835
MLA
Shimono, M., Uenaka, A., Noguchi, Y., Sato, S., Okumura, H., Nakagawa, K., Kiura, K., Tanimoto, M., Nakayama, E."Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells". International Journal of Oncology 30.4 (2007): 835-840.
Chicago
Shimono, M., Uenaka, A., Noguchi, Y., Sato, S., Okumura, H., Nakagawa, K., Kiura, K., Tanimoto, M., Nakayama, E."Identification of DR9-restricted XAGE antigen on lung adenocarcinoma recognized by autologous CD4 T-cells". International Journal of Oncology 30, no. 4 (2007): 835-840. https://doi.org/10.3892/ijo.30.4.835