Expression of interleukin (IL)-11 and IL-11 receptor in human colorectal adenocarcinoma: IL-11 up-regulation of the invasive and proliferative activity of human colorectal carcinoma cells

  • Authors:
    • Ayumi Yoshizaki
    • Toshiyuki Nakayama
    • Kazuyuki Yamazumi
    • Yuichi Yakata
    • Mitsuru Taba
    • Ichiro Sekine
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijo.29.4.869
  • Pages: 869-876
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Abstract

Previous investigations have shown that interleukin (IL)-11/IL-11 receptor α-chain (IL-11Rα), a member of the PI3K, MAPK and JAK-STAT activating family of cytokines/receptors, correlates with the regulation of tumor progression. In this study, we established the IL-11/IL-11Rα expression profile in human colorectal adenocarcinoma (CRC) and clarified its signaling pathway and role in the invasion activity of CRC cell lines. To elucidate the role of IL-11/IL-11Rα, we examined 103 cases of CRC and 24 cases of colorectal adenoma by immunohistochemistry. In addition, we investigated the invasive activity of cell signaling pathway of CRC cell lines. The IL-11Rα expression was correlated with tumor invasion and lymphatic infiltration (p<0.01, respectively). Recombinant human IL-11 (rhIL-11) promoted the migration and proliferation of HT-29 cells and activated the PI3K and p44/p42 MAPK pathways. Wortmannin, a PI3K inhibitor, and PD98059, a p44/p42 MAPK inhibitor, significantly reduced the promotion of invasion and proliferation activity by rhIL-11, respectively. In summary, the IL-11Rα expression was correlated with clinicopathological features and IL-11 promoted the invasion via the PI3K and up-regulated the proliferation via the p44/p42 MAPK in CRC cells. These findings suggested that the IL-11/IL-11R pathway plays an important role in the progression of CRC.

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October 2006
Volume 29 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Yoshizaki, A., Nakayama, T., Yamazumi, K., Yakata, Y., Taba, M., & Sekine, I. (2006). Expression of interleukin (IL)-11 and IL-11 receptor in human colorectal adenocarcinoma: IL-11 up-regulation of the invasive and proliferative activity of human colorectal carcinoma cells. International Journal of Oncology, 29, 869-876. https://doi.org/10.3892/ijo.29.4.869
MLA
Yoshizaki, A., Nakayama, T., Yamazumi, K., Yakata, Y., Taba, M., Sekine, I."Expression of interleukin (IL)-11 and IL-11 receptor in human colorectal adenocarcinoma: IL-11 up-regulation of the invasive and proliferative activity of human colorectal carcinoma cells". International Journal of Oncology 29.4 (2006): 869-876.
Chicago
Yoshizaki, A., Nakayama, T., Yamazumi, K., Yakata, Y., Taba, M., Sekine, I."Expression of interleukin (IL)-11 and IL-11 receptor in human colorectal adenocarcinoma: IL-11 up-regulation of the invasive and proliferative activity of human colorectal carcinoma cells". International Journal of Oncology 29, no. 4 (2006): 869-876. https://doi.org/10.3892/ijo.29.4.869