Additive effects of Cox-1 and Cox-2 inhibition on breast cancer in vitro

  • Authors:
    • David W. McFadden
    • Dale R. Riggs
    • Barbara J. Jackson
    • Cynthia Cunningham
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/ijo.29.4.1019
  • Pages: 1019-1023
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Abstract

We hypothesized that combined treatment with Cox-1 and Cox-2 specific inhibitiors would exhibit synergistic effects against breast cancer in vitro. Two human breast cancer cell lines (HTB26, MCF-7) were treated with catechin (Cox-1 inhibitor) or NS398 (Cox-2 inhibitor) at 100 µM as both single and combined treatments. Reductions in cell growth were observed in both cell lines at 24 and 72 h in both single and combined treatments (p<0.001). Combined treatment produced a significantly greater inhibition as compared to single agents alone. Upon cell cycle evaluation, Cox-1 and -2 antagonism increased G1 and G2 phase fractions in MCF-7 cells (p<0.001 and p<0.05 respectively). No additive changes were observed when the two agents were combined. An increase in the G2 phase was observed in the HTB26 cells when treated with NS398 alone (p<0.001). However, a decrease in the S-phase was observed when these cells were treated with NS398, as a single agent (p<0.01) or when the two agents were combined (p<0.01). The significant and additive effects exhibited by the combination of Cox-1 and -2 inhibitors and their effects on cell cycle suggest that these agents could become an effective treatment modality for carcinoma of the breast.

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October 2006
Volume 29 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
McFadden, D.W., Riggs, D.R., Jackson, B.J., & Cunningham, C. (2006). Additive effects of Cox-1 and Cox-2 inhibition on breast cancer in vitro. International Journal of Oncology, 29, 1019-1023. https://doi.org/10.3892/ijo.29.4.1019
MLA
McFadden, D. W., Riggs, D. R., Jackson, B. J., Cunningham, C."Additive effects of Cox-1 and Cox-2 inhibition on breast cancer in vitro". International Journal of Oncology 29.4 (2006): 1019-1023.
Chicago
McFadden, D. W., Riggs, D. R., Jackson, B. J., Cunningham, C."Additive effects of Cox-1 and Cox-2 inhibition on breast cancer in vitro". International Journal of Oncology 29, no. 4 (2006): 1019-1023. https://doi.org/10.3892/ijo.29.4.1019