Expression of E6/E7 mRNA from ‘high risk’ human papillomavirus in relation to CIN grade, viral load and p16INK4a

  • Authors:
    • Sonia Andersson
    • Berit Hansson
    • Ingrid Norman
    • Vera Gaberi
    • Miriam Mints
    • Anders Hjerpe
    • Frank Karlsen
    • Bo Johansson
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijo.29.3.705
  • Pages: 705-711
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Abstract

Detection of E6/E7 mRNA expression with real-time nucleic acid sequence-based amplification assay (NASBA) method (PreTect HPV-Proofer) from high-risk types of human papillomaviruses (HR-HPV) were compared with the presence of viral load, determined with quantitative real-time PCR in 80 cervical samples. Results regarding positivity and typing were in agreement using the two methods. However, there was no correlation between viral loads for HPV 16 or 18/45 and oncogene expression. Among 15 women with low grade atypia detected at a population-based cytology screening, and scored as ‘within normal limits’ according to histopathology, 14% were positive for oncogene expression, whereas 71% were HR-HPV positive. A correlation was observed between HR-HPV oncogene expression and high scores of p16INK4a positivity. Since HPV-Proofer detects full-length E6/E7 mRNA, a positive result should correlate with presence of integrated HPV, loss of HPV replication and stabilized E6/E7 full-length mRNA expression. Such expression from integrated HR-HPV generates a high and stable expression of full-length E6 proteins, which explains why a positive HPV-Proofer result was independent of viral load and correlate with high expression of p16INK4a. Thus, E6/E7 oncogene expression analysis yielded information, which is consistent with and will complement the results from a real-time PCR method in a clinical prognostic procedure.

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September 2006
Volume 29 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Andersson, S., Hansson, B., Norman, I., Gaberi, V., Mints, M., Hjerpe, A. ... Johansson, B. (2006). Expression of E6/E7 mRNA from ‘high risk’ human papillomavirus in relation to CIN grade, viral load and p16INK4a. International Journal of Oncology, 29, 705-711. https://doi.org/10.3892/ijo.29.3.705
MLA
Andersson, S., Hansson, B., Norman, I., Gaberi, V., Mints, M., Hjerpe, A., Karlsen, F., Johansson, B."Expression of E6/E7 mRNA from ‘high risk’ human papillomavirus in relation to CIN grade, viral load and p16INK4a". International Journal of Oncology 29.3 (2006): 705-711.
Chicago
Andersson, S., Hansson, B., Norman, I., Gaberi, V., Mints, M., Hjerpe, A., Karlsen, F., Johansson, B."Expression of E6/E7 mRNA from ‘high risk’ human papillomavirus in relation to CIN grade, viral load and p16INK4a". International Journal of Oncology 29, no. 3 (2006): 705-711. https://doi.org/10.3892/ijo.29.3.705