The expression of the carboxyl ester lipase gene in pancreas and pancreatic adenocarcinomas

  • Authors:
    • Reinhard Reuss
    • Susanne Aberle
    • Karin Klingel
    • Martina Sauter
    • Annette Greschniok
    • Folker Ernst Franke
    • Winfried Padberg
    • Nikolaus Blin
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/ijo.29.3.649
  • Pages: 649-654
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Abstract

Since pancreatic cancer is an aggressive and often incurable malignancy, we investigated if the carboxyl ester lipase gene (CEL) is specifically expressed in pancreatic tissues and its promoter can be used for a specific suicide gene approach. Twenty-five tumor samples, 24 samples of normal pancreatic tissue and control tissues from other organs were examined by radioactive in situ hybridization (ISH) to localize CEL mRNA. Two carcinoma samples and 6 permanent cell lines were examined by reverse transcriptase-polymerase chain reaction (RT-PCR). By ISH, we verified a strong CEL gene expression in acinar cells of the normal pancreas. A minor expression was noted in a single sample of acinar cell carcinoma and adenocarcinomas did not show any expression. By RT-PCR, no specific expression in both tested adenocarcinomas was observed. In summary, these results show that, contrary to notable expression of carboxyl ester lipase in acinar cells of normal pancreatic tissue, this lipase is not significantly active in pancreatic adenocarcinomas and thus not an apt genetic marker for diagnostic or therapeutic approaches.

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September 2006
Volume 29 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Reuss, R., Aberle, S., Klingel, K., Sauter, M., Greschniok, A., Franke, F.E. ... Blin, N. (2006). The expression of the carboxyl ester lipase gene in pancreas and pancreatic adenocarcinomas. International Journal of Oncology, 29, 649-654. https://doi.org/10.3892/ijo.29.3.649
MLA
Reuss, R., Aberle, S., Klingel, K., Sauter, M., Greschniok, A., Franke, F. E., Padberg, W., Blin, N."The expression of the carboxyl ester lipase gene in pancreas and pancreatic adenocarcinomas". International Journal of Oncology 29.3 (2006): 649-654.
Chicago
Reuss, R., Aberle, S., Klingel, K., Sauter, M., Greschniok, A., Franke, F. E., Padberg, W., Blin, N."The expression of the carboxyl ester lipase gene in pancreas and pancreatic adenocarcinomas". International Journal of Oncology 29, no. 3 (2006): 649-654. https://doi.org/10.3892/ijo.29.3.649