Dysregulated TGF-β1-induced Smad signalling occurs as a result of defects in multiple components of the TGF-β signalling pathway in human head and neck carcinoma cell lines

  • Authors:
    • Miranda Pring
    • Stephen Prime
    • E. Kenneth Parkinson
    • Ian Paterson
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/ijo.28.5.1279
  • Pages: 1279-1285
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Abstract

This study examined Smad2- and Smad3-dependent transcription in 12 human head and neck squamous cell carcinoma (HNSCC) cell lines following treatment with transforming growth factor-β1 (TGF-β1). A markedly elevated level of TGF-β1-induced Smad3 signalling was observed in one cell line (H357), whilst four cell lines (BICR31, H314, BICR56, BICR19) demonstrated absence of Smad3-dependent transcription that correlated with loss of TGF-β1 growth inhibition; TGF-β1-induced Smad2-dependent transcription was retained in two of these cell lines (H314, BICR31). Using transient expression of TGF-β signalling components and a Smad3-dependent reporter assay, we show that BICR31 and H314 had defects of Smad4, BICR56 had abnormal TβR-II and BICR19 overexpressed Smad7. The results demonstrate that deregulated TGF-β1-induced Smad signalling is common in HNSCC cell lines and can occur as a result of a variety of defects in the TGF-β signal transduction pathway.

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May 2006
Volume 28 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Pring, M., Prime, S., Parkinson, E.K., & Paterson, I. (2006). Dysregulated TGF-β1-induced Smad signalling occurs as a result of defects in multiple components of the TGF-β signalling pathway in human head and neck carcinoma cell lines. International Journal of Oncology, 28, 1279-1285. https://doi.org/10.3892/ijo.28.5.1279
MLA
Pring, M., Prime, S., Parkinson, E. K., Paterson, I."Dysregulated TGF-β1-induced Smad signalling occurs as a result of defects in multiple components of the TGF-β signalling pathway in human head and neck carcinoma cell lines". International Journal of Oncology 28.5 (2006): 1279-1285.
Chicago
Pring, M., Prime, S., Parkinson, E. K., Paterson, I."Dysregulated TGF-β1-induced Smad signalling occurs as a result of defects in multiple components of the TGF-β signalling pathway in human head and neck carcinoma cell lines". International Journal of Oncology 28, no. 5 (2006): 1279-1285. https://doi.org/10.3892/ijo.28.5.1279