Chemosensitivity prediction in esophageal squamous cell carcinoma: Novel marker genes and efficacy-prediction formulae using their expression data

  • Authors:
    • Tatsushi Shimokuni
    • Keiji Tanimoto
    • Keiko Hiyama
    • Keiko Otani
    • Megu Ohtaki
    • Jun Hihara
    • Kazuhiro Yoshida
    • Tsuyoshi Noguchi
    • Katsunobu Kawahara
    • Shoji Natsugoe
    • Takashi Aikou
    • Yasushi Okazaki
    • Yoshihide Hayashizaki
    • Yuji Sato
    • Satoru Todo
    • Eiso Hiyama
    • Masahiko Nishiyama
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/ijo.28.5.1153
  • Pages: 1153-1162
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Abstract

Esophageal cancer is a highly lethal disease and the optimal therapy remains unclear. Since adjuvant chemotherapy gives a better chance of survival, we attempted to develop a chemosensitivity prediction model to improve individual responses to therapy. Comprehensive gene expression analyses (cDNA and oligonucleotide microarrays) and MTT assay of 8 drugs in 20 KYSE squamous cell carcinoma cell lines were performed to distinguish candidate marker genes whose expression levels reproducibly correlated with cellular drug sensitivities. After confirmation with real-time RT-PCR, we performed multiple regression analyses to develop drug-sensitivity prediction formulae using the quantified expression data of selected marker genes. Using the same sets of genes, we also constructed prediction models for individual clinical responses to 5-FU-based chemotherapy using 18 cases. We selected 5 better marker genes, known as drug sensitivity determinants, identified 9 novel predictive genes for 4 of 8 anticancer drugs [5-FU, CDDP, DOX, and CPT-11 (SN-38)], and developed highly predictive formulae of in vitro sensitivities to the 4 drugs and clinical responses to 5-FU-based adjuvant chemotherapies in terms of overall and disease-free survivals. Our selected genes are likely to be effective drug-sensitivity markers and formulae using the 9 novel genes would provide advantages in prediction.

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May 2006
Volume 28 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Shimokuni, T., Tanimoto, K., Hiyama, K., Otani, K., Ohtaki, M., Hihara, J. ... Nishiyama, M. (2006). Chemosensitivity prediction in esophageal squamous cell carcinoma: Novel marker genes and efficacy-prediction formulae using their expression data. International Journal of Oncology, 28, 1153-1162. https://doi.org/10.3892/ijo.28.5.1153
MLA
Shimokuni, T., Tanimoto, K., Hiyama, K., Otani, K., Ohtaki, M., Hihara, J., Yoshida, K., Noguchi, T., Kawahara, K., Natsugoe, S., Aikou, T., Okazaki, Y., Hayashizaki, Y., Sato, Y., Todo, S., Hiyama, E., Nishiyama, M."Chemosensitivity prediction in esophageal squamous cell carcinoma: Novel marker genes and efficacy-prediction formulae using their expression data". International Journal of Oncology 28.5 (2006): 1153-1162.
Chicago
Shimokuni, T., Tanimoto, K., Hiyama, K., Otani, K., Ohtaki, M., Hihara, J., Yoshida, K., Noguchi, T., Kawahara, K., Natsugoe, S., Aikou, T., Okazaki, Y., Hayashizaki, Y., Sato, Y., Todo, S., Hiyama, E., Nishiyama, M."Chemosensitivity prediction in esophageal squamous cell carcinoma: Novel marker genes and efficacy-prediction formulae using their expression data". International Journal of Oncology 28, no. 5 (2006): 1153-1162. https://doi.org/10.3892/ijo.28.5.1153