Caspase-2: a possible trigger of apoptosis induced in A-549 tumor cells by ZnPc photodynamic treatment

  • Authors:
    • Javier Cristóbal
    • Juan C. Stockert
    • Angeles Villanueva
    • Santiago Rello-Varona
    • Angeles Juarranz
    • Magdalena Cañete
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/ijo.28.5.1057
  • Pages: 1057-1063
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Abstract

Photodynamic therapy applied to cell cultures represents a widely accepted experimental method to investigate molecular mechanisms that lead to apoptotic cell death. In this context the subcellular localization of photosensitizers seems to be a significant factor in order to determine the apoptotic pathway that could be activated. We have characterized the experimental conditions that induce apoptotic cell death in A-549 cells incubated with ZnPc and irrradiated with red light. Previously we have found that in this cell line the drug is localized in the Golgi apparatus after 3-h incubation. Indirect immunofluorescence analysis of the events that lead to apoptosis made possible the detection of caspase-2 activation in the Golgi region immediately after photodynamic treatments. A few minutes later, the morphology of this organelle starts to disrupt and just 6 h after treatment the nuclei appear affected showing the fragmented appearance typical of apoptotic cell death. From this results we assume that following the photodynamic treatment of A-549 cells with ZnPc, the activation of caspase-2 in the Golgi apparatus could begin to initiate immediately the apoptotic process.

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May 2006
Volume 28 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Cristóbal, J., Stockert, J.C., Villanueva, A., Rello-Varona, S., Juarranz, A., & Cañete, M. (2006). Caspase-2: a possible trigger of apoptosis induced in A-549 tumor cells by ZnPc photodynamic treatment. International Journal of Oncology, 28, 1057-1063. https://doi.org/10.3892/ijo.28.5.1057
MLA
Cristóbal, J., Stockert, J. C., Villanueva, A., Rello-Varona, S., Juarranz, A., Cañete, M."Caspase-2: a possible trigger of apoptosis induced in A-549 tumor cells by ZnPc photodynamic treatment". International Journal of Oncology 28.5 (2006): 1057-1063.
Chicago
Cristóbal, J., Stockert, J. C., Villanueva, A., Rello-Varona, S., Juarranz, A., Cañete, M."Caspase-2: a possible trigger of apoptosis induced in A-549 tumor cells by ZnPc photodynamic treatment". International Journal of Oncology 28, no. 5 (2006): 1057-1063. https://doi.org/10.3892/ijo.28.5.1057