Effects of PPAR and RXR ligands in semaphorin 6B gene expression of human MCF-7 breast cancer cells

  • Authors:
    • Hossam Murad
    • Philippe Collet
    • Cecile Huin-Schohn
    • Nehmann Al-Makdissy
    • Geraldine Kerjan
    • Alain Chedotal
    • Mireille Donner
    • Marie Dominique Devignes
    • Philippe Becuwe
    • Herve Schohn
    • Lionel Domenjoud
    • Michel Dauça
  • View Affiliations

  • Published online on: April 1, 2006     https://doi.org/10.3892/ijo.28.4.977
  • Pages: 977-984
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Abstract

This study tests the hypothesis that the activators of peroxisome proliferator-activated receptors (PPARs) and 9-cis-retinoic acid receptor (RXR) regulate human semaphorin 6B (Sema6B) gene expression. The human MCF-7 breast adenocarcinoma cell line was chosen because it expresses Sema6B at a high level. The Sema6B mRNA level was analyzed by RT-PCR and the semaphorin 6B protein content was determined using a polyclonal antibody that we have produced and characterized. Treatments with fenofibrate (a PPARα activator) and troglitazone (a PPARγ ligand) strongly decreased the Sema6B mRNA. The drop in Sema6B mRNA level and in protein content was more important when the treatment combined the action of fenofibrate or troglitazone and 9-cis-retinoic acid. On the other hand, no significant change was observed in the Sema6B mRNA and protein levels when the cells were exposed to the combined action of GW610742 (a PPARβ activator) and 9-cis-retinoic acid. These data suggest that PPARα/RXR and PPARγ/RXR heterodimers are involved in the regulation of Sema6B gene expression and open new perspectives concerning the participation of these nuclear receptors in cell recognition and migration.

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April 2006
Volume 28 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Murad, H., Collet, P., Huin-Schohn, C., Al-Makdissy, N., Kerjan, G., Chedotal, A. ... Dauça, M. (2006). Effects of PPAR and RXR ligands in semaphorin 6B gene expression of human MCF-7 breast cancer cells. International Journal of Oncology, 28, 977-984. https://doi.org/10.3892/ijo.28.4.977
MLA
Murad, H., Collet, P., Huin-Schohn, C., Al-Makdissy, N., Kerjan, G., Chedotal, A., Donner, M., Devignes, M. D., Becuwe, P., Schohn, H., Domenjoud, L., Dauça, M."Effects of PPAR and RXR ligands in semaphorin 6B gene expression of human MCF-7 breast cancer cells". International Journal of Oncology 28.4 (2006): 977-984.
Chicago
Murad, H., Collet, P., Huin-Schohn, C., Al-Makdissy, N., Kerjan, G., Chedotal, A., Donner, M., Devignes, M. D., Becuwe, P., Schohn, H., Domenjoud, L., Dauça, M."Effects of PPAR and RXR ligands in semaphorin 6B gene expression of human MCF-7 breast cancer cells". International Journal of Oncology 28, no. 4 (2006): 977-984. https://doi.org/10.3892/ijo.28.4.977