Significance of Id-1 up-regulation and its association with EGFR in bladder cancer cell invasion

  • Authors:
    • Yi Ding
    • Gang Wang
    • Ming Tat Ling
    • Y. C. Wong
    • Xin Li
    • Yanqun Na
    • Xiaomeng Zhang
    • Chee Wai Chua
    • Xianghong Wang
    • Dianqi Xin
  • View Affiliations

  • Published online on: April 1, 2006     https://doi.org/10.3892/ijo.28.4.847
  • Pages: 847-854
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Abstract

Epidermal growth factor receptor (EGFR) is suggested to be one of the positive factors in the invasive progression of bladder cancer. Id-1 (inhibitor of differentiation or DNA binding), a helix-loop-helix (HLH) transcription factor, was recently identified as a key factor in the EGFR signalling pathway. The aim of this study was to investigate the role of Id-1 in bladder cancer progression and its relation-ship with EGFR. Using clinical specimens from different stages of bladder cancer, immunohistochemical staining was performed to determine if Id-1 expression was positively associated with tumour staging and EGFR expression. The direct role of Id-1 in cancer cell invasion was also investigated through ectopically expressing the Id-1 gene in a RT112 bladder cancer cell line by wound closure and collagen invasion assays. To explore the therapeutic potential of targeting the Id-1 gene in the treatment of invasive bladder cancer, we studied if inactivation of the Id-1 gene through small RNA interference could lead to the suppression of invasion in a MGHU1 bladder cancer cell line. Our results showed that the up-regulation of Id-1 was associated with increased EGFR expression, clinical staging and the invasion ability of bladder cancer cells. Inactivation of Id-1 may be a potential therapeutic target to inhibit the invasion by bladder cancer cells.

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April 2006
Volume 28 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Ding, Y., Wang, G., Ling, M.T., Wong, Y.C., Li, X., Na, Y. ... Xin, D. (2006). Significance of Id-1 up-regulation and its association with EGFR in bladder cancer cell invasion. International Journal of Oncology, 28, 847-854. https://doi.org/10.3892/ijo.28.4.847
MLA
Ding, Y., Wang, G., Ling, M. T., Wong, Y. C., Li, X., Na, Y., Zhang, X., Chua, C. W., Wang, X., Xin, D."Significance of Id-1 up-regulation and its association with EGFR in bladder cancer cell invasion". International Journal of Oncology 28.4 (2006): 847-854.
Chicago
Ding, Y., Wang, G., Ling, M. T., Wong, Y. C., Li, X., Na, Y., Zhang, X., Chua, C. W., Wang, X., Xin, D."Significance of Id-1 up-regulation and its association with EGFR in bladder cancer cell invasion". International Journal of Oncology 28, no. 4 (2006): 847-854. https://doi.org/10.3892/ijo.28.4.847