Lysocellin, a metabolite of the novel drug ‘alopestatin’, induces G1 arrest and prevents cytotoxicity induced by etoposide

  • Authors:
    • Yoshinori Takahara
    • Shingo Yogosawa
    • Sakiko Maruyama
    • Noriko Watanabe
    • Hirofumi Yokoyama
    • Kazuteru Fukasawa
    • Yoshikazu Sukenaga
    • Jun Kamiyama
    • Moriatsu Izumi
    • Miki Wakada
    • Helin Zhang
    • Kaname Yoshizawa
    • Shigeyuki Kawa
    • Toshio Nikaido
    • Toshiyuki Sakai
  • View Affiliations

  • Published online on: April 1, 2006     https://doi.org/10.3892/ijo.28.4.823
  • Pages: 823-829
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Abstract

We report here that lysocellin, a polyether antibiotic from a streptomycete, induces G1 phase arrest in human osteosarcoma MG63 cells. Lysocellin up-regulates p21WAF1/Cip1 and down-regulates cyclin D1 at the mRNA level. In addition, cyclin D1 is down-regulated by the proteasome-dependent signal pathway in MG63 cells. In drug combination studies, we found that lysocellin treatment weakened the cytotoxic activity of etoposide in MG63 cells using a colony-formation assay. To study the in vivo efficacy of lysocellin, we isolated a novel compound related to lysocellin from the same streptomycete, and found that the novel drug is converted to lysocellin in vivo and decreases etoposide-induced alopecia in a neonatal rat model. We raise the possibility that this novel drug, named ‘alopestatin’, may be a promising agent against alopecia.

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April 2006
Volume 28 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Takahara, Y., Yogosawa, S., Maruyama, S., Watanabe, N., Yokoyama, H., Fukasawa, K. ... Sakai, T. (2006). Lysocellin, a metabolite of the novel drug ‘alopestatin’, induces G1 arrest and prevents cytotoxicity induced by etoposide. International Journal of Oncology, 28, 823-829. https://doi.org/10.3892/ijo.28.4.823
MLA
Takahara, Y., Yogosawa, S., Maruyama, S., Watanabe, N., Yokoyama, H., Fukasawa, K., Sukenaga, Y., Kamiyama, J., Izumi, M., Wakada, M., Zhang, H., Yoshizawa, K., Kawa, S., Nikaido, T., Sakai, T."Lysocellin, a metabolite of the novel drug ‘alopestatin’, induces G1 arrest and prevents cytotoxicity induced by etoposide". International Journal of Oncology 28.4 (2006): 823-829.
Chicago
Takahara, Y., Yogosawa, S., Maruyama, S., Watanabe, N., Yokoyama, H., Fukasawa, K., Sukenaga, Y., Kamiyama, J., Izumi, M., Wakada, M., Zhang, H., Yoshizawa, K., Kawa, S., Nikaido, T., Sakai, T."Lysocellin, a metabolite of the novel drug ‘alopestatin’, induces G1 arrest and prevents cytotoxicity induced by etoposide". International Journal of Oncology 28, no. 4 (2006): 823-829. https://doi.org/10.3892/ijo.28.4.823