Postnatal neovascularization by endothelial progenitor cells immortalized with the simian virus 40T antigen gene

  • Authors:
    • Hui-Ying Qiu
    • Yoshihiro Fujimori
    • Keisuke Nishioka
    • Nobuko Yamaguchi
    • Tomoko Hashimoto-Tamaoki
    • Ayako Sugihara
    • Nobuyuki Terada
    • Noritoshi Nagaya
    • Munetake Kanda
    • Naoya Kobayashi
    • Noriaki Tanaka
    • Karen A. Westerman
    • Philippe Leboulch
    • Hiroshi Hara
  • View Affiliations

  • Published online on: April 1, 2006     https://doi.org/10.3892/ijo.28.4.815
  • Pages: 815-821
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Abstract

Endothelial progenitor cells (EPCs) contribute to blood vessel formation in ischemic and tumorous tissues, but comprise only a small population in circulation. We attempted to immortalize putative EPCs from human cord blood. Human CD34+ cord blood cells were cultured in the presence of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF), and transfected with a retroviral vector encoding the simian virus 40 large T (SV40T) antigen. This resulted in the immortalization of cord blood cells, leading to the establishment of several cell lines. One of these lines, HYCEC-1, exhibited a phenotype characteristic of the endothelial lineage, including expression of von Willebrand factor and VEGF receptor-2 (VEGFR-2/KDR/Flk-1) and uptake of acetylated-low density lipoprotein. Flow cytometric analysis revealed that HYCEC-1 cells were strongly positive for CD31 and CD146, moderately positive for CD144, weakly positive for CD133 and CD34, and negative for CD14 and CD45. HYCEC-1 cells formed capillary-like structures on basement matrix gel in vitro. Upon transplantation into the ischemic hind limb of nude rats, HYCEC-1 cells efficiently participated in neovascularization and augmented blood flow. The immortalized HYCEC-1 cells are suggested to be a class of EPCs that can efficiently participate in postnatal neovasculogenesis in the ischemic hind limb, and may also be a useful tool for studying tumor vessel formation.

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April 2006
Volume 28 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Qiu, H., Fujimori, Y., Nishioka, K., Yamaguchi, N., Hashimoto-Tamaoki, T., Sugihara, A. ... Hara, H. (2006). Postnatal neovascularization by endothelial progenitor cells immortalized with the simian virus 40T antigen gene. International Journal of Oncology, 28, 815-821. https://doi.org/10.3892/ijo.28.4.815
MLA
Qiu, H., Fujimori, Y., Nishioka, K., Yamaguchi, N., Hashimoto-Tamaoki, T., Sugihara, A., Terada, N., Nagaya, N., Kanda, M., Kobayashi, N., Tanaka, N., Westerman, K. A., Leboulch, P., Hara, H."Postnatal neovascularization by endothelial progenitor cells immortalized with the simian virus 40T antigen gene". International Journal of Oncology 28.4 (2006): 815-821.
Chicago
Qiu, H., Fujimori, Y., Nishioka, K., Yamaguchi, N., Hashimoto-Tamaoki, T., Sugihara, A., Terada, N., Nagaya, N., Kanda, M., Kobayashi, N., Tanaka, N., Westerman, K. A., Leboulch, P., Hara, H."Postnatal neovascularization by endothelial progenitor cells immortalized with the simian virus 40T antigen gene". International Journal of Oncology 28, no. 4 (2006): 815-821. https://doi.org/10.3892/ijo.28.4.815