From molecular characteristics to cellularevents in apoptosis-resistant HL-60 cells

  • Authors:
    • Valérie Palissot
    • Hamid Morjani
    • Francis Belloc
    • Sophie Cotteret
    • Jean Dufer
    • Guy Berchem
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijo.26.3.825
  • Pages: 825-834
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Abstract

The ability to induce apoptosis in tumor cells is critical to elicit a positive response to cytotoxic chemo-therapy. In this study, we investigated the effect of the topoisomerase I inhibitors camptothecin and SN-38, known to cause an unusual form of DNA damage, on apoptotic pathways using the leukemic cell line HL-60 and its vincristine-resistant variant HL-60 VCR. Both camptothecin and SN-38 induced high levels of apoptosis in sensitive cells when compared to the multidrug-resistant ones. Interestingly, a higher BCL-2/BAX ratio was observed in HL-60 VCR at the basal state and during treatments. Moreover, these cells which did not exhibit Bcr-abl translocation or bcrp efflux pump, overexpressed topoisomerase I protein. The data provide evidence that BCL-2 protein could protect HL-60 VCR from mitochondrial membrane depolarization and block ROS production in these cells. Finally, our results suggest that dysregulation of proteins associated with DNA replication and apoptotic process could contribute to the multidrug-resistance phenotype.

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March 2005
Volume 26 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Palissot, V., Morjani, H., Belloc, F., Cotteret, S., Dufer, J., & Berchem, G. (2005). From molecular characteristics to cellularevents in apoptosis-resistant HL-60 cells. International Journal of Oncology, 26, 825-834. https://doi.org/10.3892/ijo.26.3.825
MLA
Palissot, V., Morjani, H., Belloc, F., Cotteret, S., Dufer, J., Berchem, G."From molecular characteristics to cellularevents in apoptosis-resistant HL-60 cells". International Journal of Oncology 26.3 (2005): 825-834.
Chicago
Palissot, V., Morjani, H., Belloc, F., Cotteret, S., Dufer, J., Berchem, G."From molecular characteristics to cellularevents in apoptosis-resistant HL-60 cells". International Journal of Oncology 26, no. 3 (2005): 825-834. https://doi.org/10.3892/ijo.26.3.825