IL-4 and IL-10 synergistically inhibit survival of human blood monocytes supported by GM-CSF

  • Authors:
    • Hiroyuki Miyashita
    • Naoyuki Katayama
    • Atsushi Fujieda
    • Tetsunori Shibasaki
    • Kentaro Yamamura
    • Yuka Sugimoto
    • Eri Miyata
    • Kohshi Ohishi
    • Kazuhiro Nishii
    • Masahiro Masuya
    • Hiroshi Shiku
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijo.26.3.731
  • Pages: 731-735
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Abstract

Interleukin (IL)-4 and IL-10 have a wide variety of activities in the immune system. We re-evaluated the action of IL-4 and IL-10 on human blood monocytes, myeloid dendritic cell (DC1) precursors, using a serum-free culture system. Both IL-4 and IL-10 inhibited the survival of CD14+ monocytes supported by granulocyte-macrophage colony-stimulating factor in a dose-dependent manner. When IL-4 and IL-10 were combined, they had synergistic effects at low doses and induced a profound suppression of CD14+ monocyte survival. When the optimal timing was determined, the exposure to IL-4 and IL-10 for the initial 2 days was essential for suppression of survival of CD14+ monocytes. Annexin V/propidium iodide staining indicates that the suppression of CD14+ monocyte survival induced by IL-4 and IL-10 results from apoptosis. Tumor necrosis factor-α and lipopolysaccharide abrogated the effects of IL-4 and IL-10 on CD14+ monocytes, albeit incompletely. Thus, IL-4 in synergy with IL-10 negatively regulates the survival of DC1 precursor monocytes by inducing their apoptosis, which is modulated by factors such as tumor necrosis factor-α and lipopolysaccharide. Our data suggest the primary activities of IL-4 and IL-10 in DC1-mediated immune responses.

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March 2005
Volume 26 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Miyashita, H., Katayama, N., Fujieda, A., Shibasaki, T., Yamamura, K., Sugimoto, Y. ... Shiku, H. (2005). IL-4 and IL-10 synergistically inhibit survival of human blood monocytes supported by GM-CSF. International Journal of Oncology, 26, 731-735. https://doi.org/10.3892/ijo.26.3.731
MLA
Miyashita, H., Katayama, N., Fujieda, A., Shibasaki, T., Yamamura, K., Sugimoto, Y., Miyata, E., Ohishi, K., Nishii, K., Masuya, M., Shiku, H."IL-4 and IL-10 synergistically inhibit survival of human blood monocytes supported by GM-CSF". International Journal of Oncology 26.3 (2005): 731-735.
Chicago
Miyashita, H., Katayama, N., Fujieda, A., Shibasaki, T., Yamamura, K., Sugimoto, Y., Miyata, E., Ohishi, K., Nishii, K., Masuya, M., Shiku, H."IL-4 and IL-10 synergistically inhibit survival of human blood monocytes supported by GM-CSF". International Journal of Oncology 26, no. 3 (2005): 731-735. https://doi.org/10.3892/ijo.26.3.731