Characterization of the mechanisms of electrochemotherapy in an in vitro model for human cervical cancer

  • Authors:
    • Bujian Tang
    • Li Li
    • Zhifeng Jiang
    • Yingzi Luan
    • Danrong Li
    • Wei Zhang
    • Eddie Reed
    • Qingdi Q. Li
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijo.26.3.703
  • Pages: 703-711
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Electrochemical treatment is among the most effective therapies in the management of cervical malignancy. However, the mechanism of action of this treatment remains largely unknown. Therefore, the purpose of the current project was to establish a suitable eletrochemotherapy regimen for cervical cancer and to investigate the mechanism of the therapy in an in vitro model for human cervical carcinoma. HeLa cells were used as a model for cervical cancer in this study, and the effect of electrochemical treatment on these cells was examined in four different dosage groups (5 V + 5 C, 10 V + 5 C, 5 V + 10 C and 10 V + 10 C). Our results showed that the combinations of lower voltage and higher current (5 V/10 V + 10 C) had a greater anticancer effect in this model as compared to other groups. In addition, we compared the cytotoxic effect between electrochemical treatment and different pH condition treatments in this system, and found that the efficacy of electrochemical treatment in cell killing was better than that of acidic or basic medium treatment. Moreover, we demonstrated that the efficacy of electrochemical treatment was correlated with the degree of ionization and alteration in pH scale. The electrodes were basic on the cathode side which elevated the cations K+, Ca2+ and Mg2+, while the electrodes were acidic on the anode side which reduced the anion Cl−. We also assessed the effect of electrochemical treatment on cell cycle distribution in HeLa cells and showed that the percentage of cells in the G1 phase of the cell cycle was increased (G1 arrest), while the cell population in the S phase was decreased. Furthermore, we demonstrated that the levels of the cell cycle regulator cyclin D1 expression were dramatically reduced when 5 V/10 V + 10 C treatments were applied to these cells, as determined by RT-PCR analysis. By contrast, no significant changes in the levels of cyclin B1, CDK1 or CDK4 were detected. Based on these observations, we conclude that the combination of lower voltage and higher current may be a potentially effective eletrochemotherapy regimen for cervical cancer in the clinic, and that the antitumor effect of electrochemical treatment on cervical carcinoma cells is mediated partly via regulating ionization degree, pH state and cell cycle control.

Related Articles

Journal Cover

March 2005
Volume 26 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Tang, B., Li, L., Jiang, Z., Luan, Y., Li, D., Zhang, W. ... Li, Q.Q. (2005). Characterization of the mechanisms of electrochemotherapy in an in vitro model for human cervical cancer. International Journal of Oncology, 26, 703-711. https://doi.org/10.3892/ijo.26.3.703
MLA
Tang, B., Li, L., Jiang, Z., Luan, Y., Li, D., Zhang, W., Reed, E., Li, Q. Q."Characterization of the mechanisms of electrochemotherapy in an in vitro model for human cervical cancer". International Journal of Oncology 26.3 (2005): 703-711.
Chicago
Tang, B., Li, L., Jiang, Z., Luan, Y., Li, D., Zhang, W., Reed, E., Li, Q. Q."Characterization of the mechanisms of electrochemotherapy in an in vitro model for human cervical cancer". International Journal of Oncology 26, no. 3 (2005): 703-711. https://doi.org/10.3892/ijo.26.3.703