Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro

  • Authors:
    • Mika Kijima
    • Masanao Saio
    • Guan-Feng Oyang
    • Tatsuhiko Suwa
    • Rumiko Miyauchi
    • Yasushi Kojima
    • Hisashi Imai
    • Jiro Nakagawa
    • Kenichi Nonaka
    • Naoki Umemura
    • Takashi Nishimura
    • Tsuyoshi Takami
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijo.26.3.679
  • Pages: 679-684
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The natural killer (NK) cell is one of the key cells in discriminating major histocompatibility complex (MHC) negative ‘missing-self’ target tumor cells, and interleukin 2 (IL-2) treatment was effective in inducing NK cell activation. In this study, we tried to clarify how poorly-immunogenic murine B16 melanoma could be discriminated in vivo by creating an IL-2 cDNA-transduced immunogene therapy model (B16/IL-2). In vitro study showed that IL-2 introduction did not induce MHC class I. However, immune cells depleted total tumor digest, which consisted of 90% anti-melanoma MM2-9B6-positive cells that revealed B16/IL-2 strongly, and control tumor cells (B16/mock) partially expressed MHC class I in vivo. In the B16/IL-2 model, NK cell infiltration was 10 times higher than B16/mock (7.6 versus 0.73, p=0.017). In addition, the cell surface of CD69-expressing NK cell population was increased in B16/IL-2, and the interferon gamma (IFNγ) message level in NK cells was significantly increased in B16/IL-2 (p=0.0359). Interestingly, NK cell depletion in vivo completely abolished MHC class I expression on B16/mock, and decreased MHC class I expression and T-cell infiltration in B16/IL-2. These data suggest that NK cells are not only important for missing-self recognition, but are also crucial for induction of tumor cell MHC molecule expression and play an important role in helping acquired immunity to recognize tumor cells.

Related Articles

Journal Cover

March 2005
Volume 26 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Kijima, M., Saio, M., Oyang, G., Suwa, T., Miyauchi, R., Kojima, Y. ... Takami, T. (2005). Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro. International Journal of Oncology, 26, 679-684. https://doi.org/10.3892/ijo.26.3.679
MLA
Kijima, M., Saio, M., Oyang, G., Suwa, T., Miyauchi, R., Kojima, Y., Imai, H., Nakagawa, J., Nonaka, K., Umemura, N., Nishimura, T., Takami, T."Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro". International Journal of Oncology 26.3 (2005): 679-684.
Chicago
Kijima, M., Saio, M., Oyang, G., Suwa, T., Miyauchi, R., Kojima, Y., Imai, H., Nakagawa, J., Nonaka, K., Umemura, N., Nishimura, T., Takami, T."Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro". International Journal of Oncology 26, no. 3 (2005): 679-684. https://doi.org/10.3892/ijo.26.3.679