DJ-1 restores p53 transcription activity inhibited by Topors/p53BP3

  • Authors:
    • Yumi Shinbo
    • Takahiro Taira
    • Takeshi Niki
    • Sanae M.M. Iguchi-Ariga
    • Hiroyoshi Ariga
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijo.26.3.641
  • Pages: 641-648
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Abstract

DJ-1 is a multi-functional protein that plays roles in transcriptional regulation and anti-oxidative stress, and loss of its function is thought to result in onset of Parkinson's disease. Here, we report that DJ-1 bound to Topors/p53BP3, a ring finger protein binding to both topoisomerase I and p53, in vitro and in vivo and that both proteins were colocalized in cells. DJ-1 and p53 were then found to be sumoylated by Topors in cells. It was also found that DJ-1 bound to p53 in vitro and in vivo and that colocalization with and its binding to p53 were stimulated by UV irradiation of cells. Transcription activity of p53 was found to be abrogated by Topors concomitant with sumoylation of p53 in a dose-dependent manner, and DJ-1 restored its repressed activity by releasing the sumoylated form of p53. These findings suggest that DJ-1 positively regulates p53 through Topors-mediated sumoylation.

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March 2005
Volume 26 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Shinbo, Y., Taira, T., Niki, T., Iguchi-Ariga, S.M., & Ariga, H. (2005). DJ-1 restores p53 transcription activity inhibited by Topors/p53BP3. International Journal of Oncology, 26, 641-648. https://doi.org/10.3892/ijo.26.3.641
MLA
Shinbo, Y., Taira, T., Niki, T., Iguchi-Ariga, S. M., Ariga, H."DJ-1 restores p53 transcription activity inhibited by Topors/p53BP3". International Journal of Oncology 26.3 (2005): 641-648.
Chicago
Shinbo, Y., Taira, T., Niki, T., Iguchi-Ariga, S. M., Ariga, H."DJ-1 restores p53 transcription activity inhibited by Topors/p53BP3". International Journal of Oncology 26, no. 3 (2005): 641-648. https://doi.org/10.3892/ijo.26.3.641