Identification of circulating prostate cancer cells: A challenge to the clinical implementation of molecular biology (Review)

  • Authors:
    • Denis H.J. Schamhart
    • Ruth Maiazza
    • Karl-Heinz Kurth
  • View Affiliations

  • Published online on: March 1, 2005     https://doi.org/10.3892/ijo.26.3.565
  • Pages: 565-577
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Conventional diagnosis of prostate cancer does not appear to be sensitive enough to differentiate pre-operatively between organ-confined and extracapsular disease. New technologies, arising from the field of molecular biology, have been introduced to improve diagnosis and their implementation into the clinical practice is nowadays extensively explored. In 1992, focusing on prostate cancer, the application of the highly sensitive and specific reverse transcription-polymerase chain reaction (RT-PCR) technology which amplifies predefined mRNA species was introduced. Assuming haematogenous prostate tissue-specific mRNA species to be representative for the presence of circulating prostate cancer cells, an impressive series of clinical studies, for the greater part addressing mRNA encoding for prostate-specific antigen (PSA), were performed to improve pre-operative staging (molecular staging) and prognosis of prostate cancer, and to study iatrogenic cell dissemination. In this review we summarize the efforts, concentrating on the RT-PCR methodology, to identify extracapsular prostate cancer cells in easy accessible body fluids. The substantial amount of available biological and clinical data allow an in depth illustration of the advantages, disadvantages and clinical future of this technology. The intrinsic limitations of the technology, technical as well as biological, will be addressed since these may well explain the controversies associated with its general acceptance in the clinical practice. Together with considerable improvements of the methodology to be expected in the near future, new avenues for the detection of disseminated prostate cancer cells will be subject of discussion.

Related Articles

Journal Cover

March 2005
Volume 26 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Schamhart, D.H., Maiazza, R., & Kurth, K. (2005). Identification of circulating prostate cancer cells: A challenge to the clinical implementation of molecular biology (Review). International Journal of Oncology, 26, 565-577. https://doi.org/10.3892/ijo.26.3.565
MLA
Schamhart, D. H., Maiazza, R., Kurth, K."Identification of circulating prostate cancer cells: A challenge to the clinical implementation of molecular biology (Review)". International Journal of Oncology 26.3 (2005): 565-577.
Chicago
Schamhart, D. H., Maiazza, R., Kurth, K."Identification of circulating prostate cancer cells: A challenge to the clinical implementation of molecular biology (Review)". International Journal of Oncology 26, no. 3 (2005): 565-577. https://doi.org/10.3892/ijo.26.3.565