Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number

  • Authors:
    • Shuichiro Sato
    • Yuji Noguchi
    • Hisashi Wada
    • Shoichiro Fujita
    • Shinichiro Nakamura
    • Ryo Tanaka
    • Tetsuya Nakada
    • Kosei Hasegawa
    • Kazuhiko Nakagawa
    • Fumihito Koizumi
    • Toshiro Ono
    • Kazuhiro Nouso
    • Achim Jungbluth
    • Yao-Tseng Chen
    • Lloyd J. Old
    • Yasushi Shiratori
    • Eiichi Nakayama
  • View Affiliations

  • Published online on: January 1, 2005     https://doi.org/10.3892/ijo.26.1.57
  • Pages: 57-63
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

We investigated NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and various types of cancer by quantitative real-time RT-PCR. In addition to their high expression in the testis, we observed a low expression of NY-ESO-1 mRNA in the placenta, pancreas and liver, and no expression in 12 other normal tissues. We also observed a low expression of LAGE-1a mRNA in the placenta and ovary, and marginal expression in 13 other normal tissues. In contrast to the previous finding that NY-ESO-1 and LAGE-1a mRNAs were mostly co-expressed in solid tumors, we found an independent expression of NY-ESO-1 and LAGE-1a mRNAs. NY-ESO-1 mRNA expression was mostly associated with LAGE-1a mRNA expression in esophageal and liver cancers, but not in prostate cancer. Immunohistochemistry (IHC) using NY-ESO-1-specific ES121 mAb showed that NY-ESO-1 protein was detected in 6 of 9 and 3 of 10 NY-ESO-1 mRNA-positive specimens from esophageal and liver cancers, respectively. NY-ESO-1 protein expression was correlated with the copy numbers of NY-ESO-1 mRNA. IHC was also performed using ES121 mAb and B9.8 mAb recognizing both NY-ESO-1 and LAGE-1a in 4 esophageal and 6 liver cancer specimens preferentially expressing LAGE-1a mRNA. B9.8-specific staining was observed weakly and focally in one liver cancer specimen expressing >105 copies of LAGE-1a mRNA.

Related Articles

Journal Cover

January 2005
Volume 26 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Sato, S., Noguchi, Y., Wada, H., Fujita, S., Nakamura, S., Tanaka, R. ... Nakayama, E. (2005). Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number. International Journal of Oncology, 26, 57-63. https://doi.org/10.3892/ijo.26.1.57
MLA
Sato, S., Noguchi, Y., Wada, H., Fujita, S., Nakamura, S., Tanaka, R., Nakada, T., Hasegawa, K., Nakagawa, K., Koizumi, F., Ono, T., Nouso, K., Jungbluth, A., Chen, Y., Old, L. J., Shiratori, Y., Nakayama, E."Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number". International Journal of Oncology 26.1 (2005): 57-63.
Chicago
Sato, S., Noguchi, Y., Wada, H., Fujita, S., Nakamura, S., Tanaka, R., Nakada, T., Hasegawa, K., Nakagawa, K., Koizumi, F., Ono, T., Nouso, K., Jungbluth, A., Chen, Y., Old, L. J., Shiratori, Y., Nakayama, E."Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number". International Journal of Oncology 26, no. 1 (2005): 57-63. https://doi.org/10.3892/ijo.26.1.57