YY-1 and c-Jun transcription factors participate in the repression of the human involucrin promoter

  • Authors:
    • L. M. Alvarez-Salas
    • M. L. Benitez-Hess
    • J. A. Dipaolo
  • View Affiliations

  • Published online on: January 1, 2005     https://doi.org/10.3892/ijo.26.1.259
  • Pages: 259-266
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Abstract

The human involucrin gene, which encodes a precursor of the keratinocyte cornified layer, is strongly expressed in response to differentiation stimuli. Earlier studies suggested that YY1 and components of the AP-1 family might participate in the silencing of involucrin in proliferating keratinocytes. This study shows that overexpression of either YY1 or c-Jun represses transcription of the human involucrin gene in multiplying keratinocytes. Transient overexpression and site-directed mutagenesis experiments of the involucrin 5'-non-coding region (5'-NCR) confirmed that YY1 and c-Jun repress involucrin transcription. This repression involves the distal zinc fingers of YY1 protein and the DNA binding and leucine zipper domains of c-Jun. The results with protein pull-down experiments are consistent with the hypothesis that interaction of YY1 with c-Jun is an important mechanism for involucrin repression. Cotransfection of YY1 modified the stimulatory function of mutant c-Jun proteins independently of their DNA binding capacity suggesting that interactions may be more complex in vivo. Additionally, c-Jun protein levels are affected by differentiation stimuli indicating the importance of c-Jun in the YY1 repression pathway. Thus YY1 and c-Jun have an important role in epidermal differentiation by negatively regulating the human involucrin gene.

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January 2005
Volume 26 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Alvarez-Salas, L.M., Benitez-Hess, M.L., & Dipaolo, J.A. (2005). YY-1 and c-Jun transcription factors participate in the repression of the human involucrin promoter. International Journal of Oncology, 26, 259-266. https://doi.org/10.3892/ijo.26.1.259
MLA
Alvarez-Salas, L. M., Benitez-Hess, M. L., Dipaolo, J. A."YY-1 and c-Jun transcription factors participate in the repression of the human involucrin promoter". International Journal of Oncology 26.1 (2005): 259-266.
Chicago
Alvarez-Salas, L. M., Benitez-Hess, M. L., Dipaolo, J. A."YY-1 and c-Jun transcription factors participate in the repression of the human involucrin promoter". International Journal of Oncology 26, no. 1 (2005): 259-266. https://doi.org/10.3892/ijo.26.1.259