Identification by means of cDNA microarray analyses of gene expression modifications in squamous non-small cell lung cancers as compared to normal bronchial epithelial tissue

  • Authors:
    • Myriam Remmelink
    • Tatjana Mijatovic
    • Anne Gustin
    • Anne Mathieu
    • Katja Rombaut
    • Robert Kiss
    • Isabelle Salmon
    • Christine Decaestecker
  • View Affiliations

  • Published online on: January 1, 2005     https://doi.org/10.3892/ijo.26.1.247
  • Pages: 247-258
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Abstract

The present work analyzed the gene pattern profiles in 6 squamous non-small cell lung cancers (NSCLCs) versus 4 normal lung epithelial tissues by means of cDNA microarrays. In addition to cDNA microarray analyses, quantitative RT-PCR and immunohisto-chemical analyses were used to validate some of the results obtained. Our data enabled 7 genes to be selected by looking at the genes which were detected as being expressed in all the tumors and not expressed in all the normal samples, or inversely. Additionally, 19 genes were detected as being overexpressed in the tumors when compared to the normal tissue specimens. Of these 26 genes, 16 are not yet suspected of influencing NSCLC biology. These genes are involved in cell proliferation (G2 cyclin), signal transduction (SMARCC2, TM4SF3), apoptosis (CFLAR/FLIP), cell cytoskeleton (cytokeratins-14 and 16, α-tubulin isoform 1 and S100A10), cell adhesion (JUP), invasion (cathepsins H and O) and other biological processes (OAZ1, IGHG3, SCYA5/RANTES, β-sarcoglycan and transcobalamin I). In conclusion, we identified a number of genes as being differentially expressed in squamous NSCLCs as compared to normal lung epithelial tissue. Some of these genes (such as those involved in invasion) could be used as new prognostic markers and others, like CFLAR/FLIP, could even constitute new therapeutic targets.

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January 2005
Volume 26 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Remmelink, M., Mijatovic, T., Gustin, A., Mathieu, A., Rombaut, K., Kiss, R. ... Decaestecker, C. (2005). Identification by means of cDNA microarray analyses of gene expression modifications in squamous non-small cell lung cancers as compared to normal bronchial epithelial tissue. International Journal of Oncology, 26, 247-258. https://doi.org/10.3892/ijo.26.1.247
MLA
Remmelink, M., Mijatovic, T., Gustin, A., Mathieu, A., Rombaut, K., Kiss, R., Salmon, I., Decaestecker, C."Identification by means of cDNA microarray analyses of gene expression modifications in squamous non-small cell lung cancers as compared to normal bronchial epithelial tissue". International Journal of Oncology 26.1 (2005): 247-258.
Chicago
Remmelink, M., Mijatovic, T., Gustin, A., Mathieu, A., Rombaut, K., Kiss, R., Salmon, I., Decaestecker, C."Identification by means of cDNA microarray analyses of gene expression modifications in squamous non-small cell lung cancers as compared to normal bronchial epithelial tissue". International Journal of Oncology 26, no. 1 (2005): 247-258. https://doi.org/10.3892/ijo.26.1.247