A novel C/T polymorphism in the core promoter of human de novo cytosine DNA methyltransferase 3B6 is associated with prognosis in head and neck cancer.

  • Authors:
    • Luo Wang
    • Marivonne Rodriguez
    • Edward S Kim
    • Yunling Xu
    • Neby Bekele
    • Adel K El-Naggar
    • Waun Ki Hong
    • Li Mao
    • Yun W Oh
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijo.25.4.993
  • Pages: 993-1002
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Abstract

New biomarkers for head and neck squamous cell carcinoma (HNSCC) patients are being sought to help predict disease outcome, guide treatment, and develop new treatments. In the present study, the association between a novel functional C/T polymorphism in the core promoter of cytosine DNA methyltransferase (DNMT) 3B6 and overall survival of HNSCC patients was investigated. Genomic DNA was extracted from tumor tissue and leukocytes from each HNSCC patient. We used the PCR-restriction fragment length polymorphism (PCR-RFLP) assay to determine the DNMT3B genotype. Kaplan-Meier product-limit method and univariate/multivariate Cox proportional hazard models were used to correlate DNMT3B genotype with overall survival of HNSCC patients who underwent surgical resection. There was a marked association between DNMT3B C/T polymorphism and overall survival of HNSCC patients (P=0.004). The homozygotes (CC-genotype and TT-genotype) survived significantly longer than the heterozygotes (CT-type). The multivariate Cox proportional hazard modeling showed that HNSCC patients with CT-genotype had a hazard ratio of 4.829 over patients with CC-genotype or TT-genotype. A DNMT3B C/T polymorphism has been correlated with survival differences in HNSCC patients. If validated in larger studies, this polymorphism might be used to identify deleterious patterns of gene silencing by methylation in HNSCC.

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October 2004
Volume 25 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Wang, L., Rodriguez, M., Kim, E., Xu, Y., Bekele, N., El-Naggar, A. ... Oh, Y. (2004). A novel C/T polymorphism in the core promoter of human de novo cytosine DNA methyltransferase 3B6 is associated with prognosis in head and neck cancer.. International Journal of Oncology, 25, 993-1002. https://doi.org/10.3892/ijo.25.4.993
MLA
Wang, L., Rodriguez, M., Kim, E., Xu, Y., Bekele, N., El-Naggar, A., Hong, W., Mao, L., Oh, Y."A novel C/T polymorphism in the core promoter of human de novo cytosine DNA methyltransferase 3B6 is associated with prognosis in head and neck cancer.". International Journal of Oncology 25.4 (2004): 993-1002.
Chicago
Wang, L., Rodriguez, M., Kim, E., Xu, Y., Bekele, N., El-Naggar, A., Hong, W., Mao, L., Oh, Y."A novel C/T polymorphism in the core promoter of human de novo cytosine DNA methyltransferase 3B6 is associated with prognosis in head and neck cancer.". International Journal of Oncology 25, no. 4 (2004): 993-1002. https://doi.org/10.3892/ijo.25.4.993