Enhancement of apoptosis in salivary gland cancer cells by the combination of oral fluoropyrimidine anticancer agent (S-1) and radiation

  • Authors:
    • Koji Harada
    • Shin-Ichi Kawaguchi
    • Supriatno
    • Tomitaro Onoue
    • Hideo Yoshida
    • Mitsunobu Sato
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijo.25.4.905
  • Pages: 905-911
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Abstract

S-1 is a new oral antineoplastic agent which can inhibit cell growth and induces apoptosis in certain types of cancer cells including gastric carcinomas, colorectal cancers and salivary gland cancers, but its effect on response of tumor cells to radiation has not been clarified yet. We have reported that S-1 can sensitize human oral cancer cells to radiation, and that S-1 in combination with radiation can exert remarkable effects on decreasing clonogenic survival and in vivo tumor growth. Here, we demonstrate the mechanism of apoptosis enhancing activity by the combination treatment of S-1 and radiation in salivary gland cancer cells. S-1 in combination with radiation have a remarkable effect on decreasing in vitro cell growth. In addition, the combined treatment of S-1 and radiation resulted in an increased DNA fragmentation by detecting Hoechst 33258 staining. Moreover, apoptosis of the cells by combined treatment of S-1 and radiation was associated with reactive oxygen/nitrogen species generation and the activation of caspase-8, -9 and -3. These results indicate that S-1 in combination with radiation can markedly enhance apoptosis of salivary gland cancer cells, and the combined therapy of S-1 and radiation are currently leading to the design of clinical studies.

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October 2004
Volume 25 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Harada, K., Kawaguchi, S., Supriatno, , Onoue, T., Yoshida, H., & Sato, M. (2004). Enhancement of apoptosis in salivary gland cancer cells by the combination of oral fluoropyrimidine anticancer agent (S-1) and radiation. International Journal of Oncology, 25, 905-911. https://doi.org/10.3892/ijo.25.4.905
MLA
Harada, K., Kawaguchi, S., Supriatno, , Onoue, T., Yoshida, H., Sato, M."Enhancement of apoptosis in salivary gland cancer cells by the combination of oral fluoropyrimidine anticancer agent (S-1) and radiation". International Journal of Oncology 25.4 (2004): 905-911.
Chicago
Harada, K., Kawaguchi, S., Supriatno, , Onoue, T., Yoshida, H., Sato, M."Enhancement of apoptosis in salivary gland cancer cells by the combination of oral fluoropyrimidine anticancer agent (S-1) and radiation". International Journal of Oncology 25, no. 4 (2004): 905-911. https://doi.org/10.3892/ijo.25.4.905