Modulation of the transactivation function of nuclear factor-kappaB by lipopolysaccharide in RAW264.7 macrophages.

  • Authors:
    • Sang Won Jung
    • Mi Hee Lee
    • Yong Chan Kim
    • Sang-Gi Paik
    • Yung Hyun Choi
    • Young Sang Kim
    • Kwang Il Kang
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijo.25.4.1081
  • Pages: 1081-1088
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Abstract

In macrophages, nuclear factor kappa B (NF-kappaB) activation has important roles for the regulation of an inducible nitric oxide synthase (iNOS), several pro-inflammatory cytokines, and anti-apoptotic proteins. In order to analyze the transactivation process of NF-kappaB by lipopolysaccharide (LPS), we used the GAL4-NF-kappaB-p65 fusion protein. This chimeric NF-kappaB is activated transcriptionally only if NF-kappaB transactivation domain is active. With this system, we found that LPS can enhance the transactivation of GAL-NF-kappaB-p65 subunit independent of DNA binding ability and inhibitor of kappaB (IkappaB) regulation. Interestingly, this transactivation by LPS was eliminated with the treatment of U0126, specific inhibitor of mitogen-activated protein kinase (MAPK) kinases (MEKs) 1/2 which has little effect on NF-kappaB activation. We also investigated the effect of inhibitors of apoptosis (IAPs), which might be involved in LPS responses and c-Jun amino terminal kinase (JNKs) activation, on the transactivation of GAL-NF-kappaB-p65. The cIAP1, cIAP2 and XIAP could enhance the NF-kappaB transcription and the chimeric NF-kappaB-p65 transactivation. However, survivin decreased the NF-kappaB transcription and did not influence significantly the chimeric NF-kappaB-p65 transactivation. Taken together, LPS-dependent NF-kappaB transactivation may be involved in extracellular signal-regulated kinase (ERK) pathway and IAPs.

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October 2004
Volume 25 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Jung, S., Lee, M., Kim, Y., Paik, S., Choi, Y., Kim, Y., & Kang, K. (2004). Modulation of the transactivation function of nuclear factor-kappaB by lipopolysaccharide in RAW264.7 macrophages.. International Journal of Oncology, 25, 1081-1088. https://doi.org/10.3892/ijo.25.4.1081
MLA
Jung, S., Lee, M., Kim, Y., Paik, S., Choi, Y., Kim, Y., Kang, K."Modulation of the transactivation function of nuclear factor-kappaB by lipopolysaccharide in RAW264.7 macrophages.". International Journal of Oncology 25.4 (2004): 1081-1088.
Chicago
Jung, S., Lee, M., Kim, Y., Paik, S., Choi, Y., Kim, Y., Kang, K."Modulation of the transactivation function of nuclear factor-kappaB by lipopolysaccharide in RAW264.7 macrophages.". International Journal of Oncology 25, no. 4 (2004): 1081-1088. https://doi.org/10.3892/ijo.25.4.1081