Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.

  • Authors:
    • Hirohiko Okamura
    • Kaya Yoshida
    • Eiko Sasaki
    • Hiroyuki Morimoto
    • Tatsuji Haneji
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijo.25.4.1031
  • Pages: 1031-1038
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Abstract

In this study, the expression and transcriptional regulation of the multidrug resistance-1 (MDR1) gene in multidrug-resistant SCCTF cells and -sensitive SCCKN cells derived from human squamous carcinoma were investigated. RT-PCR revealed that mdr1 mRNA was highly expressed in SCCTF cells while it was under the limit of detection in SCCKN cells. With an electrophoretic mobility shift assay using the mdr1 promoter region, a DNA-protein complex was detected strongly in SCCTF cells, but weakly in SCCKN cells. Incubation of the DNA-protein complex with an anti-NF-Y antibody caused a supershift in the migration to a position near the origin of the gel. Chromatin immunoprecipitation assay with an anti-NF-Y antibody showed that NF-Y binds to mdr1 promoter in SCCTF cells. The mdr1 promoter region including its NF-Y binding sequence was cloned into the luciferase reporter plasmid pGL3-basic vector, and this vector was used to transfect SCCTF and SCCKN cells. The luciferase assay showed that the inverted CCAAT sequence in the mdr1 promoter region is involved in the positive regulation of mdr1 promoter activity. NF-YA protein was expressed at higher levels in SCCTF cells than that in SCCKN cells. Hoechst dye staining also showed that MDR1 protein acts more effectively as an efflux pump in SCCTF cells than that in SCCKN cells.

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October 2004
Volume 25 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., & Haneji, T. (2004). Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.. International Journal of Oncology, 25, 1031-1038. https://doi.org/10.3892/ijo.25.4.1031
MLA
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., Haneji, T."Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.". International Journal of Oncology 25.4 (2004): 1031-1038.
Chicago
Okamura, H., Yoshida, K., Sasaki, E., Morimoto, H., Haneji, T."Transcription factor NF-Y regulates mdr1 expression through binding to inverted CCAAT sequence in drug-resistant human squamous carcinoma cells.". International Journal of Oncology 25, no. 4 (2004): 1031-1038. https://doi.org/10.3892/ijo.25.4.1031