CRIPTO-1: a novel target for therapeutic intervention in human carcinoma.

  • Authors:
    • Nicola Normanno
    • Antonella De Luca
    • Monica R Maiello
    • Caterina Bianco
    • Mario Mancino
    • Luigi Strizzi
    • Claudio Arra
    • Fortunato Ciardiello
    • Sudhir Agrawal
    • David S Salomon
  • View Affiliations

  • Published online on: October 1, 2004     https://doi.org/10.3892/ijo.25.4.1013
  • Pages: 1013-1033
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Abstract

Evidence suggests that CRIPTO-1 (CR-1) might be involved in the pathogenesis of human carcinoma. In the present study, we have screened the expression of CR-1 mRNA and protein in a wide panel of human cancer cell lines by using reverse transcriptase (RT)-PCR, real-time PCR and immunocytochemistry. Results of these experiments demonstrate that CR-1 is expressed in several, different carcinoma types. The anchorage-independent growth of colon, ovarian, lung and breast carcinoma cells was significantly inhibited by treatment with anti-CR-1 second generation antisense oligonucleotides. Similar results were obtained with anti-transforming growth factor alpha (TGF-alpha) and anti-amphiregulin (AR) antisense oligonucleotides. Treatment of carcinoma cells with CR-1 antisense oligonucleotides resulted in a significant reduction in the levels of expression of CR-1 mRNA and protein, and in the levels of activation of Akt. Finally, oral administration of either CR-1, AR or TGF-alpha antisense oligonucleotides produced a significant reduction in the growth of GEO colon carcinoma xenografts in nude mice that was associated with a reduction in the levels of expression of the target proteins. Taken together, these data strongly suggest that CR-1 might represent a novel target for therapeutic intervention in different carcinoma types.

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October 2004
Volume 25 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Normanno, N., De Luca, A., Maiello, M., Bianco, C., Mancino, M., Strizzi, L. ... Salomon, D. (2004). CRIPTO-1: a novel target for therapeutic intervention in human carcinoma.. International Journal of Oncology, 25, 1013-1033. https://doi.org/10.3892/ijo.25.4.1013
MLA
Normanno, N., De Luca, A., Maiello, M., Bianco, C., Mancino, M., Strizzi, L., Arra, C., Ciardiello, F., Agrawal, S., Salomon, D."CRIPTO-1: a novel target for therapeutic intervention in human carcinoma.". International Journal of Oncology 25.4 (2004): 1013-1033.
Chicago
Normanno, N., De Luca, A., Maiello, M., Bianco, C., Mancino, M., Strizzi, L., Arra, C., Ciardiello, F., Agrawal, S., Salomon, D."CRIPTO-1: a novel target for therapeutic intervention in human carcinoma.". International Journal of Oncology 25, no. 4 (2004): 1013-1033. https://doi.org/10.3892/ijo.25.4.1013