Identification of a functional peroxisome proliferator activated receptor response element in the 3' untranslated region of the human bcl-2 gene

  • Authors:
    • Brent D. Butts
    • Nhan L. Tran
    • Margaret M. Briehl
  • View Affiliations

  • Published online on: May 1, 2004     https://doi.org/10.3892/ijo.24.5.1305
  • Pages: 1305-1310
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Abstract

Peroxisome proliferator activated receptors are nuclear hormone receptors that regulate the expression of genes containing a peroxisome proliferator activated receptor response element. We report here that the human bcl-2 gene contains a functional peroxisome proliferator activated receptor response element in the 3' untranslated region. Peroxisome proliferator activated receptor γ bound the human bcl-2 peroxisome proliferator activated receptor response element in gel shift assays and co-transfection of this receptor led to increased luciferase activity from a reporter plasmid containing the human bcl-2 peroxisome proliferator activated receptor response element. Examination of peroxisome proliferator activated receptor γ-transfected cells demonstrated an increased amount of bcl-2 message compared to empty vector-transfected cells. Confocal analyses confirmed that more Bcl-2 protein was present in peroxisome proliferator activated receptor γ-transfected cells compared to control-transfected cells. The functionality of the increased Bcl-2 protein was examined using resistance to bile salt-induced apoptosis as the endpoint. Peroxisome proliferator activated receptor γ-transfected cells were almost twice as resistant as control-transfected cells. These data show that PPARγ can mediate transcription of bcl-2, resulting in an increase in Bcl-2 protein and protection from apoptosis. We discuss these findings with regards to their potential implications for colon carcinogenesis.

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May 2004
Volume 24 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Butts, B.D., Tran, N.L., & Briehl, M.M. (2004). Identification of a functional peroxisome proliferator activated receptor response element in the 3' untranslated region of the human bcl-2 gene. International Journal of Oncology, 24, 1305-1310. https://doi.org/10.3892/ijo.24.5.1305
MLA
Butts, B. D., Tran, N. L., Briehl, M. M."Identification of a functional peroxisome proliferator activated receptor response element in the 3' untranslated region of the human bcl-2 gene". International Journal of Oncology 24.5 (2004): 1305-1310.
Chicago
Butts, B. D., Tran, N. L., Briehl, M. M."Identification of a functional peroxisome proliferator activated receptor response element in the 3' untranslated region of the human bcl-2 gene". International Journal of Oncology 24, no. 5 (2004): 1305-1310. https://doi.org/10.3892/ijo.24.5.1305