Decreased levels of osteopontin and bone sialoprotein II are correlated with reduced proliferation, colony formation, and migration of GFP-MDA-MB-231 cells

  • Authors:
    • Hassan Adwan
    • Tobias Bäuerle
    • Yousef Najajreh
    • Victoria Elazer
    • Gershon Golomb
    • Martin R. Berger
  • View Affiliations

  • Published online on: May 1, 2004     https://doi.org/10.3892/ijo.24.5.1235
  • Pages: 1235-1244
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Abstract

MDA-MB-231 human breast cancer cells transfected with GFP were used as model to determine the reduction in proliferation, colony formation, and migration in response to agents with anti-metastatic properties. These agents consisted of antisense oligonucleotides (ASOs) directed against osteopontin (OPN), bone sialoprotein II (BSP II), and osteonectin (ON), as well as an antibody directed against BSP II. A bisphosphonate derivative (ibandronate) and an alkylphosphocholine (erucylphospho-NNN-trimethylpropanolamine; ErPC3) were used as positive controls. The ASOs directed against OPN, BSP II and ON suppressed the expression of their respective target proteins by 81%, 74% and 69%, respectively. They were barely but significantly active in inhibiting the proliferation, but intermediately to highly active in inhibiting the colony formation and migration of GFP-MDA-MB-231 breast cancer cells. The antibody against human BSP II was significantly more active than all ASOs used and was equally active or even surpassed the activity of ibandronate and ErPC3 in all three assays. The results obtained suggest a specific anti-metastatic activity of this antibody as well as of the ASOs found effective in decreasing OPN and BSP II expression.

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May 2004
Volume 24 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Adwan, H., Bäuerle, T., Najajreh, Y., Elazer, V., Golomb, G., & Berger, M.R. (2004). Decreased levels of osteopontin and bone sialoprotein II are correlated with reduced proliferation, colony formation, and migration of GFP-MDA-MB-231 cells. International Journal of Oncology, 24, 1235-1244. https://doi.org/10.3892/ijo.24.5.1235
MLA
Adwan, H., Bäuerle, T., Najajreh, Y., Elazer, V., Golomb, G., Berger, M. R."Decreased levels of osteopontin and bone sialoprotein II are correlated with reduced proliferation, colony formation, and migration of GFP-MDA-MB-231 cells". International Journal of Oncology 24.5 (2004): 1235-1244.
Chicago
Adwan, H., Bäuerle, T., Najajreh, Y., Elazer, V., Golomb, G., Berger, M. R."Decreased levels of osteopontin and bone sialoprotein II are correlated with reduced proliferation, colony formation, and migration of GFP-MDA-MB-231 cells". International Journal of Oncology 24, no. 5 (2004): 1235-1244. https://doi.org/10.3892/ijo.24.5.1235