Necrotic cell death induced by photodynamic treatment of human lung adenocarcinoma A-549 cells with palladium(II)-tetraphenylporphycene

  • Authors:
    • M. Cañete
    • C. Ortega
    • A. Gavaldá
    • J. Cristobal
    • A. Juarranz
    • S. Nonell
    • J. Teixidó
    • J. I. Borrell
    • A. Villanueva
    • S. Rello
    • J. C. Stockert
  • View Affiliations

  • Published online on: May 1, 2004     https://doi.org/10.3892/ijo.24.5.1221
  • Pages: 1221-1228
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Abstract

In this study we describe photodamaging and photokilling effects of palladium(II)-tetraphenylporphycene (PdTPPo) (previously incorporated into dipalmitoylphosphatidylcholine liposomes) on the human lung adenocarcinoma A-549 cell line. No dark cytotoxicity was found when the drug was applied at 10−6 M or 5x10−7 M for 1 or 18 h, respectively. After 1-h treatment with 10−7 M or 5x10−7 M PdTPPo followed by red light irradiation for variable times, dose-dependent lethal effects were observed in A-549 cells. Apoptosis was not found after the above photodynamic treatments or under even milder sublethal conditions. In contrast to HeLa cells subjected to PdTPPo photosensitization where either apoptosis or necrosis were induced, morphological analysis and electrophoretical DNA pattern of A-549 cells always revealed a clearly necrotic death mechanism. However, A-549 cells died by apoptosis after serum and L-glutamine deprivation, indicating that only the photodynamically induced apoptosis was inhibited. Immunofluorescent labeling revealed that microtubules and actin microfilaments were immediately and strongly damaged by photodynamic treatments with PdTPPo. No metaphase arrest and/or mitotic alterations were observed after phototreatments. Present results show that the cell type plays a fundamental role in relation to the apoptotic or necrotic response to photosensitization, and that cytoskeletal components are important targets implicated in cell death processes.

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May 2004
Volume 24 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Cañete, M., Ortega, C., Gavaldá, A., Cristobal, J., Juarranz, A., Nonell, S. ... Stockert, J.C. (2004). Necrotic cell death induced by photodynamic treatment of human lung adenocarcinoma A-549 cells with palladium(II)-tetraphenylporphycene. International Journal of Oncology, 24, 1221-1228. https://doi.org/10.3892/ijo.24.5.1221
MLA
Cañete, M., Ortega, C., Gavaldá, A., Cristobal, J., Juarranz, A., Nonell, S., Teixidó, J., Borrell, J. I., Villanueva, A., Rello, S., Stockert, J. C."Necrotic cell death induced by photodynamic treatment of human lung adenocarcinoma A-549 cells with palladium(II)-tetraphenylporphycene". International Journal of Oncology 24.5 (2004): 1221-1228.
Chicago
Cañete, M., Ortega, C., Gavaldá, A., Cristobal, J., Juarranz, A., Nonell, S., Teixidó, J., Borrell, J. I., Villanueva, A., Rello, S., Stockert, J. C."Necrotic cell death induced by photodynamic treatment of human lung adenocarcinoma A-549 cells with palladium(II)-tetraphenylporphycene". International Journal of Oncology 24, no. 5 (2004): 1221-1228. https://doi.org/10.3892/ijo.24.5.1221