Immunohistochemical analysis and mutational analyses of β-catenin, Axin family and APC genes in hepatocellular carcinomas

  • Authors:
    • Yasuyo Ishizaki
    • Satoshi Ikeda
    • Masahiko Fujimori
    • Yosuke Shimizu
    • Takeshi Kurihara
    • Toshiyuki Itamoto
    • Akira Kikuchi
    • Masazumi Okajima
    • Toshimasa Asahara
  • View Affiliations

  • Published online on: May 1, 2004     https://doi.org/10.3892/ijo.24.5.1077
  • Pages: 1077-1083
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Abstract

Several lines of evidence show that the development of hepatocellular carcinoma (HCC) requires an accumulation of genetic alterations. However, molecular mechanism in HCC carcinogenesis remains unsolved. A total of 89 HCC samples were analyzed in this study to determine how alterations in the Wnt signaling pathway associate with the carcinogenesis of HCC. β-catenin immunohistochemistry showed positive nuclear staining in 24 (27.0%) of the 89 HCC samples, indicating the existence of alterations in the Wnt signaling pathway in those 24 HCC samples. Mutations in the β-catenin, Axin1 and Axin2 genes were detected in 10 (41.7%), 13 (54.2%) and 9 (37.5%) of the 24 β-catenin-positive samples, respectively, but no mutation was detected in the APC gene. In conclusion, in addition to mutations in the β-catenin gene, mutations in the Axin1 and Axin2 genes may alter the Wnt signaling pathway, resulting in accumulation of β-catenin.

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May 2004
Volume 24 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Ishizaki, Y., Ikeda, S., Fujimori, M., Shimizu, Y., Kurihara, T., Itamoto, T. ... Asahara, T. (2004). Immunohistochemical analysis and mutational analyses of β-catenin, Axin family and APC genes in hepatocellular carcinomas. International Journal of Oncology, 24, 1077-1083. https://doi.org/10.3892/ijo.24.5.1077
MLA
Ishizaki, Y., Ikeda, S., Fujimori, M., Shimizu, Y., Kurihara, T., Itamoto, T., Kikuchi, A., Okajima, M., Asahara, T."Immunohistochemical analysis and mutational analyses of β-catenin, Axin family and APC genes in hepatocellular carcinomas". International Journal of Oncology 24.5 (2004): 1077-1083.
Chicago
Ishizaki, Y., Ikeda, S., Fujimori, M., Shimizu, Y., Kurihara, T., Itamoto, T., Kikuchi, A., Okajima, M., Asahara, T."Immunohistochemical analysis and mutational analyses of β-catenin, Axin family and APC genes in hepatocellular carcinomas". International Journal of Oncology 24, no. 5 (2004): 1077-1083. https://doi.org/10.3892/ijo.24.5.1077