STAT3 and MAPK in human lung cancer tissues and suppression of oncogenic growth by JAB and dominant negative STAT3

  • Authors:
    • Yukio Seki
    • Noriko Suzuki
    • Munehisa Imaizumi
    • Takashi Iwamoto
    • Noriyasu Usami
    • Yuichi Ueda
    • Michinari Hamaguchi
  • View Affiliations

  • Published online on: April 1, 2004     https://doi.org/10.3892/ijo.24.4.931
  • Pages: 931-934
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Abstract

To search for the signaling events in lung carcinoma relevant to its tumorigenesis, we investigated the phosphorylation of MAPK and STAT3 in human lung carcinoma tissues and their paired normal tissues. Although relative amounts of MAPK levels differed among the cases examined, no clear difference in MAPK levels was observed between tumor tissues and their paired normal tissues. Of 79 cases examined, only 7 cases (8.9%) showed tumor-specific phosphorylation of MAPK, whereas 41 cases (52%) showed more than 2-fold lower levels of MAPK phosphorylation in tumor tissues. In contrast to MAPK, 42 cases (53%) showed tumor-specific increase in STAT3 expression. In addition, 15 cases (19%) showed tumor-specific increase of STAT3 phosphorylation and 51 cases (65%) had STAT3 phosphorylation proportional to its expression. Moreover, exogenous expression of either JAB or dominant negative STAT3 in lung carcinoma cell lines led to the suppression of STAT3 phosphorylation and the drastic reduction of anchorage- independent growth of the cells. Taken together, our results suggest that JAK-STAT3 signaling has a pivotal role for oncogenic growth of lung carcinoma cells.

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April 2004
Volume 24 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Seki, Y., Suzuki, N., Imaizumi, M., Iwamoto, T., Usami, N., Ueda, Y., & Hamaguchi, M. (2004). STAT3 and MAPK in human lung cancer tissues and suppression of oncogenic growth by JAB and dominant negative STAT3. International Journal of Oncology, 24, 931-934. https://doi.org/10.3892/ijo.24.4.931
MLA
Seki, Y., Suzuki, N., Imaizumi, M., Iwamoto, T., Usami, N., Ueda, Y., Hamaguchi, M."STAT3 and MAPK in human lung cancer tissues and suppression of oncogenic growth by JAB and dominant negative STAT3". International Journal of Oncology 24.4 (2004): 931-934.
Chicago
Seki, Y., Suzuki, N., Imaizumi, M., Iwamoto, T., Usami, N., Ueda, Y., Hamaguchi, M."STAT3 and MAPK in human lung cancer tissues and suppression of oncogenic growth by JAB and dominant negative STAT3". International Journal of Oncology 24, no. 4 (2004): 931-934. https://doi.org/10.3892/ijo.24.4.931