Down-regulation of matrix-invasive potential of human liver cancer cells by type I interferon and a histone deacetylase inhibitor sodium butyrate

  • Authors:
    • Fumihiko Kaneko
    • Hidetsugu Saito
    • Yoshimasa Saito
    • Kanji Wakabayashi
    • Nobuhiro Nakamoto
    • Shinichiro Tada
    • Hidekazu Suzuki
    • Satoshi Tsunematsu
    • Naoki Kumagai
    • Hiromasa Ishii
  • View Affiliations

  • Published online on: April 1, 2004     https://doi.org/10.3892/ijo.24.4.837
  • Pages: 837-845
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Abstract

We have demonstrated anti-proliferation and anti-metastasis effects of both interferon-α and a histone deacetylase inhibitor, sodium butyrate, on human liver cancer cell lines. In this study, invasive ability of human liver cancer cell lines through the matrix-coated membrane was examined and inhibitory effect of interferon-α and sodium butyrate was investigated. Among six human liver cancer cell lines, HLE and HLF showed high invasive ability using the Matrigel invasion assay. This invasion ability was significantly inhibited by pretreatment of the cells with 1000 IU/ml of interferon-α or 2 mM of sodium butyrate. Gelatin zymography and the matrix metalloproteinase-2 and -9 activity assay showed that these two cell lines produce active- and pro-matrix metalloproteinase-2 and -9, and their activity was significantly reduced by pretreatment with both agents. Real-time quantitative reverse transcription-polymerase chain reaction showed decrease in matrix metalloproteinase-1 mRNA levels by pretreatment with both agents, but mRNA levels of tissue inhibitor of matrix metalloproteinase-1 and -2 were differently modulated by interferon-α and sodium butyrate. These results suggest that interferon-α and sodium butyrate reduce a chance of invasion and metastasis of human liver cancer cells by inhibiting matrix metalloproteinase activity, although its inhibitor is differently regulated.

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April 2004
Volume 24 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Kaneko, F., Saito, H., Saito, Y., Wakabayashi, K., Nakamoto, N., Tada, S. ... Ishii, H. (2004). Down-regulation of matrix-invasive potential of human liver cancer cells by type I interferon and a histone deacetylase inhibitor sodium butyrate. International Journal of Oncology, 24, 837-845. https://doi.org/10.3892/ijo.24.4.837
MLA
Kaneko, F., Saito, H., Saito, Y., Wakabayashi, K., Nakamoto, N., Tada, S., Suzuki, H., Tsunematsu, S., Kumagai, N., Ishii, H."Down-regulation of matrix-invasive potential of human liver cancer cells by type I interferon and a histone deacetylase inhibitor sodium butyrate". International Journal of Oncology 24.4 (2004): 837-845.
Chicago
Kaneko, F., Saito, H., Saito, Y., Wakabayashi, K., Nakamoto, N., Tada, S., Suzuki, H., Tsunematsu, S., Kumagai, N., Ishii, H."Down-regulation of matrix-invasive potential of human liver cancer cells by type I interferon and a histone deacetylase inhibitor sodium butyrate". International Journal of Oncology 24, no. 4 (2004): 837-845. https://doi.org/10.3892/ijo.24.4.837