Relationship between the expression of extracellular signal-regulated kinase 1/2 and the dissociation of pancreatic cancer cells: Involvement of ERK1/2 in the dissociation status of cancer cells

  • Authors:
    • Xiaodong Tan
    • Hiroshi Egami
    • Shinji Ishikawa
    • Takashi Kurizaki
    • Yasuhiro Tamori
    • Eiji Takai
    • Masahiko Hirota
    • Michio Ogawa
  • View Affiliations

  • Published online on: April 1, 2004     https://doi.org/10.3892/ijo.24.4.815
  • Pages: 815-820
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Abstract

Mitogen-activated protein kinase kinase 2 (MEK2), the upstream kinase of extracellular signal-regulated kinase 1/2 (ERK1/2) was previously isolated as cancer cell dissociation related factor. In this study, to further clarify the regulatory mechanism of cancer cell dissociation, two hamster (PC-1.0 and PC-1) and human (Capan-2 and AsPC-1) pancreatic cancer cell lines were analyzed immunocytochemically with anti-ERK1, anti-ERK2 and anti-phosphorylated ERK1/2 (p-ERK1/2) antibodies. U0126 (a MEK1/2 inhibitor) significantly suppressed ERK2 and p-ERK1/2 expressions in PC-1.0 and AsPC-1 cells (P<0.05). Cancer cell dissociation factor (DF) markedly induced ERK2 and p-ERK1/2 expressions in PC-1 and Capan-2 cells (P<0.05), and the induced ERK2 and p-ERK1/2 expressions were inhibited by subsequent U0126-treatment (P<0.05). Simultaneously, light microscopic images showed that DF clearly induced cell dissociation in PC-1 and Capan-2 cells, while U0126-treatment induced cell aggregation in these pancreatic cancer cells. ERK2 activation is closely involved in cell dissociation of pancreatic cancer cells.

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April 2004
Volume 24 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Tan, X., Egami, H., Ishikawa, S., Kurizaki, T., Tamori, Y., Takai, E. ... Ogawa, M. (2004). Relationship between the expression of extracellular signal-regulated kinase 1/2 and the dissociation of pancreatic cancer cells: Involvement of ERK1/2 in the dissociation status of cancer cells. International Journal of Oncology, 24, 815-820. https://doi.org/10.3892/ijo.24.4.815
MLA
Tan, X., Egami, H., Ishikawa, S., Kurizaki, T., Tamori, Y., Takai, E., Hirota, M., Ogawa, M."Relationship between the expression of extracellular signal-regulated kinase 1/2 and the dissociation of pancreatic cancer cells: Involvement of ERK1/2 in the dissociation status of cancer cells". International Journal of Oncology 24.4 (2004): 815-820.
Chicago
Tan, X., Egami, H., Ishikawa, S., Kurizaki, T., Tamori, Y., Takai, E., Hirota, M., Ogawa, M."Relationship between the expression of extracellular signal-regulated kinase 1/2 and the dissociation of pancreatic cancer cells: Involvement of ERK1/2 in the dissociation status of cancer cells". International Journal of Oncology 24, no. 4 (2004): 815-820. https://doi.org/10.3892/ijo.24.4.815