Induction of the early response protein EGR-1 in human tumour cells after ionizing radiation is correlated with a reduction of repair of lethal lesions and an increase of repair of sublethal lesions

  • Authors:
    • Nicolaas A.P. Franken
    • Rosemarie Ten Cate
    • Chris Van Bree
    • Jaap Haveman
  • View Affiliations

  • Published online on: April 1, 2004     https://doi.org/10.3892/ijo.24.4.1027
  • Pages: 1027-1031
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Abstract

The role of EGR-1 in potentially lethal damage repair (PLDR) was studied. Induction of the early response protein EGR-1 and survival after ionizing radiation of two human tumour cell lines after culturing for 48 h in serum-deprived medium was investigated. The glioblastoma cell line (Gli-6) and a lung carcinoma cell line (SW-1573) were selected as these cell lines differ considerably in the degree of PLD repair after radiation. In both cell lines induction of EGR-1 protein was observed between 30-120 min after treatment with 10 Gy in serum-deprived cultures. In cells growing in medium with normal serum no induction of EGR-1 was observed. No difference in EGR-1 expression levels between the two cell lines was detected. Linear-Quadratic analysis of the survival curves showed a much larger difference between the values of α after immediate and delayed plated cells of the cultures in normal serum as compared to cells cultured in serum-deprived medium. The cells cultured in serum-deprived medium showed much larger difference between the values of β. This indicates that induction of EGR-1 is correlated with a reduction of repair of lethal lesions (PLDRα) and with an increase of repair of sublethal lesions (PLDRβ).

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April 2004
Volume 24 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Franken, N.A., Ten Cate, R., Van Bree, C., & Haveman, J. (2004). Induction of the early response protein EGR-1 in human tumour cells after ionizing radiation is correlated with a reduction of repair of lethal lesions and an increase of repair of sublethal lesions. International Journal of Oncology, 24, 1027-1031. https://doi.org/10.3892/ijo.24.4.1027
MLA
Franken, N. A., Ten Cate, R., Van Bree, C., Haveman, J."Induction of the early response protein EGR-1 in human tumour cells after ionizing radiation is correlated with a reduction of repair of lethal lesions and an increase of repair of sublethal lesions". International Journal of Oncology 24.4 (2004): 1027-1031.
Chicago
Franken, N. A., Ten Cate, R., Van Bree, C., Haveman, J."Induction of the early response protein EGR-1 in human tumour cells after ionizing radiation is correlated with a reduction of repair of lethal lesions and an increase of repair of sublethal lesions". International Journal of Oncology 24, no. 4 (2004): 1027-1031. https://doi.org/10.3892/ijo.24.4.1027