Modulation of Matrix metalloproteinase-1 in nasopharyngeal cancer cells by photoactivation of hypericin

  • Authors:
    • Hongyan Du
    • Malini Olivo
    • Ratha Mahendran
    • Boon-Huat Bay
  • View Affiliations

  • Published online on: March 1, 2004     https://doi.org/10.3892/ijo.24.3.657
  • Pages: 657-662
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Abstract

Singlet oxygen (1O2) generated by ultraviolet A irradiation has been reported to induce cytokine-dependent Matrix metalloproteinase-1 (MMP-1) expression in dermal fibroblasts. In the present study, we analyzed the effect of hypericin-based photodynamic therapy (PDT) on MMP-1 expression in two nasopharyngeal cancer (NPC) cell lines and an animal tumor model. MMP-1 protein and mRNA expression were evaluated by Western blot analysis and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) respectively. Photoactivation of hypericin, a polycyclic phenanthroperylenedione, elicited an increase in MMP-1 protein and mRNA expression in well differentiated HK1 and poorly differentiated CNE-2 NPC cells in vitro. Similarly, there was up-regulation of MMP1 mRNA expression in hypericin-PDT-treated NPC/HK1-tumors. To our knowledge, this is the first time that modulation of MMP-1 expression has been demonstrated as a photodynamic effect of hypericin in NPC cells. This has clinical implications as MMP-1 is known to play an essential role in the biological process of matrix remodeling.

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March 2004
Volume 24 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Du, H., Olivo, M., Mahendran, R., & Bay, B. (2004). Modulation of Matrix metalloproteinase-1 in nasopharyngeal cancer cells by photoactivation of hypericin. International Journal of Oncology, 24, 657-662. https://doi.org/10.3892/ijo.24.3.657
MLA
Du, H., Olivo, M., Mahendran, R., Bay, B."Modulation of Matrix metalloproteinase-1 in nasopharyngeal cancer cells by photoactivation of hypericin". International Journal of Oncology 24.3 (2004): 657-662.
Chicago
Du, H., Olivo, M., Mahendran, R., Bay, B."Modulation of Matrix metalloproteinase-1 in nasopharyngeal cancer cells by photoactivation of hypericin". International Journal of Oncology 24, no. 3 (2004): 657-662. https://doi.org/10.3892/ijo.24.3.657