Bcl-2 targeting siRNA expressed by a T7 vector system inhibits human tumor cell growth in vitro

  • Authors:
    • Lori Holle
    • Labri Hicks
    • Wendy Song
    • Eric Holle
    • Thomas Wagner
    • Xianzhong Yu
  • View Affiliations

  • Published online on: March 1, 2004     https://doi.org/10.3892/ijo.24.3.615
  • Pages: 615-621
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Abstract

A T7 promoter driven siRNA expression vector system (Bcl-2/T7) that targets Bcl-2 mRNA in MCF-7 human cancer cells was designed in the present study. In the presence of prebound T7 RNA polymerase, successful expression of Bcl-2 siRNA as well as its function was demonstrated via cell proliferation assays, Bcl-2 Elisa, and TUNEL assay. MCF-7 breast cancer cells transfected with Bcl-2/T7 show decreased levels of Bcl-2 expression at the protein level as well as decreased cell proliferation. Also, the number of apoptotic cells was increased in cells expressing Bcl-2 siRNA. Previous studies have shown that Bcl-2 levels are increased in a large number of different types of cancer. Therefore, the ability of Bcl-2/T7 to produce functional Bcl-2 siRNA in breast cancer cells suggests a potential role for this delivery system in cancer gene therapy.

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March 2004
Volume 24 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Holle, L., Hicks, L., Song, W., Holle, E., Wagner, T., & Yu, X. (2004). Bcl-2 targeting siRNA expressed by a T7 vector system inhibits human tumor cell growth in vitro. International Journal of Oncology, 24, 615-621. https://doi.org/10.3892/ijo.24.3.615
MLA
Holle, L., Hicks, L., Song, W., Holle, E., Wagner, T., Yu, X."Bcl-2 targeting siRNA expressed by a T7 vector system inhibits human tumor cell growth in vitro". International Journal of Oncology 24.3 (2004): 615-621.
Chicago
Holle, L., Hicks, L., Song, W., Holle, E., Wagner, T., Yu, X."Bcl-2 targeting siRNA expressed by a T7 vector system inhibits human tumor cell growth in vitro". International Journal of Oncology 24, no. 3 (2004): 615-621. https://doi.org/10.3892/ijo.24.3.615